The Possible Potential of Isolation Cancer Stem Cells from MCF7 Cell Line | ||||
| Mansoura Journal of Biology | ||||
| Volume 71, Issue 4, December 2024, Page 51-56 PDF (1.17 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjb.2024.447302 | ||||
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| Authors | ||||
| Dina Hafez Hussein Ali* 1; Amira Awadalla2; Ahmed Shokeir2; Amoura M. Abou El-Nag1 | ||||
| 1Faculty of Science, Mansoura University | ||||
| 2Center of Excellence for Genome and Cancer Research, Urology and Nephrology Center, Mansoura University, Mansoura 35516, Egypt | ||||
| Abstract | ||||
| Breast cancer represent the second cause of death in women after lung cancer. Scientists studied breast cancer in main fields genetically and epigenetically in order to understand and defeat it. Anticancer drugs were not efficient enough due to cancer recurrence which was discovered the reason beyond it which is a small population of cells that have the ability to self-renewal and cause tumorgenicity called cancer stem cells (CSCs). Understanding CSCs and its mechanisms allow us discovering the mechanism of recurrence Particularly, mammospheres that are responsible of cancer recurrence. Aim of the work is Studying the possible potential of isolation these mammosphers and percentage that would provide a great model to reveal CSCs mechanisms. In this study we cultured MCF7 cell line and exposed it into specific conditions that only CSC stem cell will survive in. Cell line was cultured in serum-free DMEM-F12 supplemented with 0.4% bovine serum albumin, 20 ng/ml epidermal growth factor (EGF), 5 mg/ml insulin, 10 ng/ml basic fibroblast growth factor (bFGF) Then incubated for one week without any disturbances. After one week, cells were checked and mammospheres appeared clearly, pictured and characterized by accurate measurement flow cytometry CD44+/CD24- which indicated that the purity of MCF-7 cancer stem cells in the mammospheres is above 98% after being cultured in serum-free medium for 1 week. The data suggest that culture of mammospheres of these metastatic cells would provide a very suitable model to investigate how responsive tumorigenic stem cells are to therapeutic drugs. | ||||
| Keywords | ||||
| Cancer; MCF7 Cell Line; Cancer Stem Cells; Mammospheres; Flow Cytometry | ||||
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