NIGELLA SATIVA LOADED PLGA NANOPARTICLES: A PROMISING ALTERNATIVE THERAPEUTIC AGENT AGAINST CHRONIC SCHISTOSOMIASIS MANSONI | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 55, Issue 2, August 2025, Page 225-232 PDF (282.6 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2025.447399 | ||||
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| Authors | ||||
AMANY ABD EL-RAHEEM ABD EL-LAAH1; MOHAMED ESSA MARGHANY2; NAGWA MOHAMED EL-SAWY3; HESHAM EBRAHIM OSMAN  4
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| 1Department of Medical Parasitology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||||
| 2Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt | ||||
| 3Department of Biochemistry, Faculty of Science, Sohag University, Egypt | ||||
| 4Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt. | ||||
| Abstract | ||||
| Praziquantel (PZQ) alone didn't improve histopathological damage of chronic schistosomia- sis. Nigella sativa decreased the egg granuloma severity and histopathological changes in schistosomiasis. PLGA is regarded as an ideal polymer for drug delivery systems. This study evaluated the effect of NSO, NSO-loaded PLGA nanoparticles alone and combined with PZQ on egg granuloma histopathology in S. mansoni Swiss Albino mice A total of 48 mice were divided into 6 groups of 8 mice each: Control negative: neither infected nor treated; control positive: infected and not treated; NSO mice: treated with NSO; NP1 mice: tr eated with NSO/PLGANPs; NP2 mice: treated with a half dose of NSO/ PLGANPs; and NPP mice: treated with PZQ and half dose of NSO/PLGANPs. All mice were sacrificed for histopa- thological evaluation of Liver egg granuloma number and diameter, oogram pattern, type and state of egg granuloma in all mice groups The results showed that NPP mice caused highest number of dead ova (91.63±1.41) follo wed by NP1 mice (14.88±2.03). Also, it caused the highest reduction in number (72.92%) and diameter (42.01%) of egg granuloma, followed by NP1 mice (67.53% & 34.42% respec- tively). The fibro-cellular granuloma was markedly increased from 15% in control positive to 63% & 43% in NPP & NP1 mice respectively. NPP mice showed the highest number of dead eggs (58%) than NP1 ones (28%) as compared to other groups and hydrobic hepatocytes de generation was improved in all treated mice, with fewer inflammatory infiltrates in the liver. | ||||
| Keywords | ||||
| NSO; PLGA; Schistosoma mansoni; Egg Granuloma; Histopathology | ||||
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