CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT
ABDEL-RAHMAN, S., HASSAN, T., BAKIR, H., OTHMAN, R., KHALIFA, M., OMRAN, E. (2025). CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT. EKB Journal Management System, 55(2), 251-258. doi: 10.21608/jesp.2025.447404
SALMA M. ABDEL-RAHMAN; TASNEEM M. HASSAN; HANAA Y. BAKIR; RAGAA A. OTHMAN; MERVAT M. KHALIFA; EMAN KH. OMRAN. "CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT". EKB Journal Management System, 55, 2, 2025, 251-258. doi: 10.21608/jesp.2025.447404
ABDEL-RAHMAN, S., HASSAN, T., BAKIR, H., OTHMAN, R., KHALIFA, M., OMRAN, E. (2025). 'CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT', EKB Journal Management System, 55(2), pp. 251-258. doi: 10.21608/jesp.2025.447404
ABDEL-RAHMAN, S., HASSAN, T., BAKIR, H., OTHMAN, R., KHALIFA, M., OMRAN, E. CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT. EKB Journal Management System, 2025; 55(2): 251-258. doi: 10.21608/jesp.2025.447404

CHARACTERIZATION OF CAPILLARIA PHILIPPINENSIS DIFFERENT ANTIGENS AND FISH CAPILLARIA SPP. ADULT WORM ANTIGEN BY SDS-PAGE AND WESTERN BLOT

Journal of the Egyptian Society of Parasitology
Volume 55, Issue 2, August 2025, Page 251-258  XML PDF (252.06 K)
Document Type: Original Article
DOI: 10.21608/jesp.2025.447404
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Authors
SALMA M. ABDEL-RAHMAN1; TASNEEM M. HASSAN1; HANAA Y. BAKIR1; RAGAA A. OTHMAN2; MERVAT M. KHALIFA1; EMAN KH. OMRAN3
1Department of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt.
2Department of Medical Parasitology, Faculty of Medicine, Assiut University, Egypt
3Department of Medical Parasitology Faculty of Medicine, Sohag University, Egypt
Abstract
Intestinal capillariasis is an emerging zoonotic disease. It can lead to life-threatening protein
losing enteropathy if not diagnosed early and treated promptly. This study clarified the antige- 
nic profiles and immunogenicity of C. philippinensis from adult, egg, coproantigen, and fish 
Capillaria spp. adult antigen using SDS-PAGE and Western Blot (WB) techniques against C. 
philippinensis sera. The electrophoretic profiles showed that most proteins were common in all 
samples at 25.6, 42, 57, & 70 kDa, but some were stage-specific. Immunoblot analysis showed 
that patient sera recognized and reacted with all examined stages. This indicated that antigenic 
components of different stages of C. philippinensis, and the common components among all 
life stages improved parasite immunodiagnosis. Also, the results showed that human sera from 
patients with intestinal capillariasis recognized the antigenic pattern of fish Capillaria spp., 
indicated that potential cross-reactivity could develop more accessible and cost-effective diag- 
nostic tools, mainly in regions where C. philippinensis-specific antigens in non-endemic areas. 
Keywords
Human C. philippinensis; Fish Capillaria spp; SDS-PAGE; Immunoblot
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