EVALUATION OF THE EFFECT OF NITROFURANTOIN ON EXPERIMENTAL CRYPTOSPORIDIUM PARVUM INFECTION IN IMMUNOSUPPRESSED MICE | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 55, Issue 2, August 2025, Page 269-279 PDF (579.34 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2025.447406 | ||||
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| Authors | ||||
| ASMAA M. BARAKAT1; DALIA S. ASHOUR1; REDA H. ELBAKARY2; WESAM M. A. ELGENDY1 | ||||
| 1Department Medical Parasitology, Faculty of Medicine, Tanta University, Egypt | ||||
| 2Department of Histology and Cell Biology, Faculty of Medicine, Tanta University, Egypt | ||||
| Abstract | ||||
| Cryptosporidium parvum, a worldwide zoonotic parasite and in immunosuppressed patients can be fatal. Nitazoxanide® (NTZ) is effective in immunocompetent patients, but unsatisfactory in im munosuppressed ones. This study evaluated nitrofurantoin® (NF) as an anti-cryptosporidial drug in immunosuppressed mice. A total of 100 immunosuppressed mice were divided into 5 groups of 20 mice each: GI: neither infected nor treated, GII: infected non-treated, GIII: infected and NTZ (250mg/kg/day) treated, GIV: infected and NF 100mg/kg/day) treated, and GV: infected and NTZ & NF treated. All drugs were given orally on 4th day post infection (P.I.) for 7 consecutive days, and then mice were gent- ly sacrificed for parasitological, histopathological, ultra-structural and biochemical assessment af- ter one and two weeks post treatment (i.e. 11th & 18th day P.I.) GV showed the highest oocysts reduction count (65.7%, & 80%), followed by GIV (48.6%, & 59.8%) but, GIII showed the least effect (25.4%, & 30.2%) on 11th, & 18th day P.I. respectively. Histopathological and TEM examinations showed marked restoration of normal intestinal struct- ure in GV, followed by significant improvement in GIV, but GIII showed partial improvement with persistent dysplastic changes in enterocytes and marked ultra-structural abnormalities. Both NTZ &NF showed significant reduction of MDA level, however, NF was more effective in elev- ating GSH level than NTZ. Both NF & NTZ restored normal liver levels of SGOT & SGPT, compared to significantly increase positive control, normal kidneys levels of urea & creatinine. | ||||
| Keywords | ||||
| C. parvum; Immunosuppressed mice; Nitrofurantoin; Nitazoxanide; Evaluation | ||||
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