Impact of Probiotics Loaded Nanoemulsion Versus Free Probiotics on Experimentally Induced Ulcerative Colitis in Adult Male Albino Rats: Histological Study | ||
| Egyptian Journal of Histology | ||
| Articles in Press, Accepted Manuscript, Available Online from 14 August 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2025.395837.2288 | ||
| Authors | ||
| Wafaa Abd El-Azeem Abdou Boughdady1; Dina Hisham Mohamed2; Sarah Mosaad Amer* 3 | ||
| 11 Anatomy department, College of Medicine, Jouf University, Sakaka, Saudi Arabia. 2 Histology department, Faculty of Medicine, Cairo University, Cairo, Egypt. | ||
| 2Histology department, Faculty of Medicine, Cairo University | ||
| 3Histology department , Faculty of Medicine, Cairo University,Cairo, Egypt | ||
| Abstract | ||
| Background and objectives: Ulcerative colitis (UC) is a common inflammatory bowel disease (IBD). Probiotics have proven their efficacy in the regeneration of intestinal mucosa; however, they have low bioavailability. Dietary nanoforms provide new approaches for improving bioavailability & safety of dietary supplements. This study aimed to explore the effect of probiotics loaded thyme essential oil nano-emulsion (TEON) in comparison with that of free probiotics on acute UC provoked by acetic acid in rats. Materials and Methods: This study included 40 adult male albino rats classified as 4 groups: Group I (Control), Group II (Ulcerative colitis): received a single intrarectal injection of 2 ml of 3% acetic acid, Group III (Probiotics treated): UC was induced in rats then rats received probiotics orally at a dose of 1.0 × 1010 CFU/kg/day . Group IV (Probiotics-TEON treated): UC was induced in rats then rats received probiotics- TEON orally at a dose of 5 mg/kg/day of TEON loaded with probiotics (1.0 × 1010 CFU/kg/day). All rats were sacrificed after 7 days. Tissue malondialdehyde (MDA) and catalase (CAT) levels were measured. Histological, morphometric and statistical studies were done. Results: Group II showed disrupted colonic wall structure. There were elevated MDA and decreased CAT levels compared to all other groups. There was a marked reduction in Alcian blue-positive reaction for goblet cells. The NF-kB showed strong positive immune reaction in the lining epithelial cells and in the inflammatory cells of the lamina propria. The α-SMA revealed decreased immune reaction in the pericryptal myofibroblasts and the muscularis mucosa. Groups III & IV showed restoration of the normal histological, biochemical and morphometric parameters. Moreover, group IV showed the best results in all parameters. Conclusion: Probiotics-TEON showed better therapeutic effects than the free probiotics on ulcerative colitis. | ||
| Keywords | ||
| Ulcerative Colitis; Free probiotics; Thyme essential oil nano-emulsion; NF-kB; α-SMA | ||
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