Confirmation between Microscopy and Pcr Technique for Differentiation between Entamoeba Histolytica and Entamoeba Dispar among Children in Duhok City, Iraq | ||||
| Ain Shams Medical Journal | ||||
| Volume 76, Issue 2, June 2025, Page 465-471 PDF (355.51 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asmj.2025.312623.1298 | ||||
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| Author | ||||
Shameeran Salman Ismael  
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| Department of Medical Laboratory Sciences, College of Health Sciences, Duhok University, Duhok, Iraq | ||||
| Abstract | ||||
| Aims: The current study aimed to confirm between microscopy and PCR techniques to differentiate between Entamoeba histolytica and Entamoeba dispar and to determine the prevalence of amoebiasis among children in Duhok City, Iraq. Materials and Methods: We conducted this study from June to August 2021. A total of 300 fresh stool samples were collected from children with a history of diarrhea/dysentery from both sexes. Results: Depending on the microscopic examination of stool samples, the prevalence rate of amoebiasis among children in the Duhok Province was high (34.0%). A higher prevalence rate was recorded according to gender in girls than in boys (18.0% & 16.0%) respectively, and among the age groups reported a high prevalence rate in the age groups between (4-6 years & 7-9 years) (15.67% & 12.33%), respectively. The lowest infection rate was recorded in the age groups (1-3 years and 10-12 years) (4.0% and 2.0%). This variation was statistically not significant at p < 0.05. Finally, the PCR results show that similar results were recorded positive for Entamoeba histolytica (92.0%). By microscopy, just a few samples were positive with Entamoeba histolytica, and by PCR, they were negative for Entamoeba histolytica and positive for Entamoeba dispar (8.0%), and one sample was found positive with Entamoeba histolytica and Entamoeba dispar. Conclusion: To lower the prevalence rate of infection among the community by supplying clean water, a suitable sanitation system, and supporting efforts to educate people about health. | ||||
| Keywords | ||||
| Amoebiasis; entamoeba histolytica; entamoeba dispar; microscopy; PCR; prevalence | ||||
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