Comparison of Actinobacterial Rhizosphere Communities in Agricultural Soil of Beni-Suef, Egypt, using Cultural and Non-Cultural Methods
Sallam, A., Tagyan, A., Mahmoud, E., Hozayen, W., Alkhalifah, D., Hozzein, W. (2025). Comparison of Actinobacterial Rhizosphere Communities in Agricultural Soil of Beni-Suef, Egypt, using Cultural and Non-Cultural Methods. EKB Journal Management System, (), 17-28. doi: 10.21608/cat.2025.380043.1378
Abdel-Aal A. Sallam; Aya I. Tagyan; Esraa Mahmoud; Walaa G. Hozayen; Dalal Hussien M. Alkhalifah; Wael N. Hozzein. "Comparison of Actinobacterial Rhizosphere Communities in Agricultural Soil of Beni-Suef, Egypt, using Cultural and Non-Cultural Methods". EKB Journal Management System, , , 2025, 17-28. doi: 10.21608/cat.2025.380043.1378
Sallam, A., Tagyan, A., Mahmoud, E., Hozayen, W., Alkhalifah, D., Hozzein, W. (2025). 'Comparison of Actinobacterial Rhizosphere Communities in Agricultural Soil of Beni-Suef, Egypt, using Cultural and Non-Cultural Methods', EKB Journal Management System, (), pp. 17-28. doi: 10.21608/cat.2025.380043.1378
Sallam, A., Tagyan, A., Mahmoud, E., Hozayen, W., Alkhalifah, D., Hozzein, W. Comparison of Actinobacterial Rhizosphere Communities in Agricultural Soil of Beni-Suef, Egypt, using Cultural and Non-Cultural Methods. EKB Journal Management System, 2025; (): 17-28. doi: 10.21608/cat.2025.380043.1378

Comparison of Actinobacterial Rhizosphere Communities in Agricultural Soil of Beni-Suef, Egypt, using Cultural and Non-Cultural Methods

Catrina: The International Journal of Environmental Sciences
Articles in Press, Accepted Manuscript, Available Online from 17 August 2025  XML PDF (1.22 MB)
Document Type: Original Article
DOI: 10.21608/cat.2025.380043.1378
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Authors
Abdel-Aal A. Sallam1; Aya I. Tagyan email orcid 2; Esraa Mahmoud3; Walaa G. Hozayen4; Dalal Hussien M. Alkhalifah5; Wael N. Hozzeinorcid 6
1Basic Science Department, Faculty of Oral and Dental Medicine, Nahda University, Beni-Suef, Egypt.
2Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
3Genetics Department, Faculty of Agriculture, Beni-Suef University, Beni-Suef , Egypt
4Department of Biochemistry, Faculty of science, Beni-Suef University, Beni-Suef 62511, Egypt
5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
6Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Abstract
The agricultural soil microbiome in Beni-Suef, Egypt, plays a crucial role in crop health and adaptation. However, the diversity and function of its microbial communities, particularly Actinobacteria, key contributors to soil health and nutrient cycling, remain largely underexplored. To investigate microbial diversity, ten rhizosphere soil samples were collected from wheat plants across five distinct regions of Beni-Suef. Both culture-dependent and culture-independent (metagenomic) methods were employed. The culture-independent analysis (metagenomic analysis) focused on identifying dominant Actinobacterial orders, while cultured isolates were identified by 16S rRNA gene sequencing. Metagenomic analysis revealed that Acidimicrobiales dominated the Actinobacterial community (60.0% to 88.0%), followed by Actinomycetales (32.7%-75.6%), Nitriliruptorales (1.5%-4.0%), and Solirubrobacterales (2.0%-6.0%). Ten Actinobacterial isolates obtained through culturing were identified as members of the genera Streptomyces, Saccharothrix, Saccharopolyspora, Nocardiopsis, and Actinocorallia. Data obtained from this study demonstrates that Actinobacterial community structure varies significantly across agricultural sites in Beni-Suef, reflecting the influence of local environmental conditions. Our findings underscore the ecological importance of Actinobacteria in maintaining soil functionality and highlight the value of integrating culture-dependent and -independent methods for comprehensive microbial community analysis.
Keywords
Actinobacterial diversity; Agricultural soil; Metagenomics; Culture-dependent method; Rhizosphere microbiome
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