Effect of Gamma Irradiation on Escherichia coli Isolated from Milk and Dairy Products | ||||
| Menoufia Veterinary Medical Journal | ||||
| Volume 1, Issue 2, July 2025, Page 1-16 PDF (1.07 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmmj.2025.406549.1054 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Asmaa Adel Shalaby 
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| Bacteriology, Immunology and Mycology Department Faculty Veterinary Medicine, Menoufia University | ||||
| Abstract | ||||
| Escherichia coli (E. coli) is one of the pathogens that can cause foodborne diseases and can cause serious health problems. In the current study, a total of 100 random samples of raw milk (n= 50), cheese (n= 25), and yogurt (n= 25) were collected from different shops and supermarkets at Menoufia Governorate, Egypt. E. coli was isolated from 32 examined samples and subjected to biochemical and serological tests for identification. Ten out of 32 isolates tested to antimicrobial susceptibility, were highly resistant for penicillin, chloramphenicol, co-trimoxazole (sulpha, trimethoprim) (100%), followed by cotrimoxazole25 (90%), ampicillin (70%) and cefotaxime (70%). The examined isolates were highly sensitive to gentamicin (90%), linezolid (90%) and kanamycin (80%), and moderately sensitive to streptomycin (60%). Bacteria employ several methods to resist antibiotics, primarily by altering their structure or function to prevent the drug from working effectively. Gamma irradiation is a non-thermal processing technology that is used as a method of preserving foods in several countries and can be used to eliminate food-borne pathogens. Three multidrug resistance (MDR) E. coli isolates were exposed to gamma irradiation at doses of 0, 0.5, 1, 2, 4, 8 KGy, then tested for the effect of Gamma Irradiation (antimicrobial susceptibility, bacterial cell mass, total E. coli count, antimicrobial activity test of E. coli and PCR). Gamma Irradiation was found to have a significant effect that led to decreased total E. coli count, bacterial cell mass and changed resistance of some strains tested for dfrA and floR genes by PCR. | ||||
| Keywords | ||||
| Escherichia coli; Gamma Irradiation; PCR; dfrA gene; floR gene | ||||
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