Evaluation genetic association between TLR4-D299G polymorphism and tonsillectomy patients infected with Streptococcus pyogenes | ||
| Microbial Biosystems | ||
| Article 11, Volume 10, Issue 3, September 2025, Pages 72-80 PDF (478.38 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mb.2025.354366.1236 | ||
| Author | ||
| Najlaa M Abdullah D. AL-Oqaili* | ||
| Department of Medical Biotechnology, College of Biotechnology, University of Al-Qadisiyah, Iraq | ||
| Abstract | ||
| Tonsillitis is currently a global health problem affecting thousands of people whether acute or chronic for which about 30% undergo surgical intervention (tonsillectomy). The aim of this study is to genetically diagnose Streptococcus pyogenes causing tonsillitis and determine the relationship between TLR4-D299G(A/G) (rs498679) gene polymorphism in patients with eradicated tonsillitis and healthy individuals. Forty tissue samples were collected from patients of different ages and genders who underwent tonsillectomy at Diwaniyah Teaching Hospital-Iraq. Meanwhile, information was collected from blood samples of the same source from patients who underwent operation for the purpose of extracting spy1258 gene and 40 blood samples from healthy individuals. The genotype of SNP TLR4-D299G(A/G) (rs4986790) was determined using T-ARMS-PCR. The results of our current study proved that the main cause of tonsillitis is S. pyogenes, with the presence of spy1258 gene (70%), in relation with the SNP TLR4- D299G (rs4986790). The homozygous genotype GG was (27.5%) for patients and (7.5%) for healthy controls. The genotype AA for patients and healthy group was (52.5%, 75.0%), respectively while genotype A/G in patients and healthy group was (20.0%,17.5%) respectively. The current study concludes that heterozygous genotype A/G is an insignificant risk factor, which means that patients with the homozygous genotype GG were about five times more likely to develop tonsillitis than those with other genotypes. | ||
| Keywords | ||
| Tonsillectomy; S. pyogenes; Spy1258 gene; TLR4-D2996G; rs 4986790(A/G) SNP | ||
|
Statistics Article View: 73 PDF Download: 19 |
||
