Antibacterial activity of iron oxide nanoparticles against MDR Klebsiella pneumoniae isolated from diabetic foot infections | ||
| Microbial Biosystems | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 September 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mb.2025.338623.1207 | ||
| Authors | ||
| Nihad J. Kadhom* 1; Nawfal H. Aldujaili2 | ||
| 1Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq | ||
| 2Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq. | ||
| Abstract | ||
| This study investigated the antibacterial potential of biosynthesized iron oxide nanoparticles against multidrug-resistant Klebsiella pneumoniae isolated from diabetic foot infections. Seventy-five clinical samples were collected between October 2023 and January 2024 from two hospitals in Hilla Province. Iron oxide nanoparticles were produced using Bacillus mesophilus (isolate N10) and characterized by UV–Vis spectroscopy, AFM, FESEM, XRD, and FTIR, showing sizes ranging from 9 to 57 nm and a characteristic FTIR peak at 589.78 cm−1. The nanoparticles demonstrated strong inhibitory effects, with zones of 23.2 mm against K. pneumoniae and 23 mm against K. oxytoca at 1000 µg/ml, along with significant anti-biofilm activity (up to 98.9% inhibition). They also exhibited antioxidant and anti-hemolytic properties without detectable toxicity to human cells. These findings suggest that biosynthesized iron oxide nanoparticles from Bacillus mesophilus could provide a safe and effective approach for managing MDR bacterial infections, especially in diabetic foot cases. | ||
| Keywords | ||
| FEO Nanoparticles; Bacteria; Biosynthesis Antioxidant; Nanotechnology; MDR Klebsiella Pneumoniae | ||
|
Statistics Article View: 78 |
||
