Optimized Bioremediation of Methyl Orange Dye and Pathogenic Bacteria from Wastewater Using Pomegranate Peel: Antibacterial and Adsorption Studies
Metwally, A., Awad, E. (2025). Optimized Bioremediation of Methyl Orange Dye and Pathogenic Bacteria from Wastewater Using Pomegranate Peel: Antibacterial and Adsorption Studies. EKB Journal Management System, (), -. doi: 10.21608/ejchem.2025.377884.11705
A Metwally; Esraa Awad. "Optimized Bioremediation of Methyl Orange Dye and Pathogenic Bacteria from Wastewater Using Pomegranate Peel: Antibacterial and Adsorption Studies". EKB Journal Management System, , , 2025, -. doi: 10.21608/ejchem.2025.377884.11705
Metwally, A., Awad, E. (2025). 'Optimized Bioremediation of Methyl Orange Dye and Pathogenic Bacteria from Wastewater Using Pomegranate Peel: Antibacterial and Adsorption Studies', EKB Journal Management System, (), pp. -. doi: 10.21608/ejchem.2025.377884.11705
Metwally, A., Awad, E. Optimized Bioremediation of Methyl Orange Dye and Pathogenic Bacteria from Wastewater Using Pomegranate Peel: Antibacterial and Adsorption Studies. EKB Journal Management System, 2025; (): -. doi: 10.21608/ejchem.2025.377884.11705

Optimized Bioremediation of Methyl Orange Dye and Pathogenic Bacteria from Wastewater Using Pomegranate Peel: Antibacterial and Adsorption Studies

Egyptian Journal of Chemistry
Articles in Press, Accepted Manuscript, Available Online from 26 August 2025  XML
Document Type: Original Article
DOI: 10.21608/ejchem.2025.377884.11705
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Authors
A Metwally email 1; Esraa Awad2
1Department of Spectroscopy, Physics Research Institute, NRC
2Botany Department, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, Egyptز
Abstract
This study investigated the dual functionality of pomegranate peel (PP) as a low-cost biosorbent and antimicrobial agent for treating industrial wastewater contaminated with methyl orange (MO) dye and pathogenic bacteria. Experimental work optimized the process through three key routes: (1) antibacterial assays of acetone, ethanol-acid, and aqueous PP extracts against B. cereus, E. coli, and S. aureus via agar well diffusion and MIC determination; (2) dye adsorption and bacterial removal studies using dry PP powder, with removal efficiency quantified by UV-Vis spectroscopy; (3) statistical optimization of dye removal via Central Composite Design (CCD) to model interaction between pH (2-9), contact time (20-120 min), adsorbent concentration (0.1-0.5 g/L), and dye concentration (2-8 ppm). Results demonstrated highest dye removal percentages of 93% at optimal conditions of 120 min contact time, pH of 9, adsorbent conc. of 0.1 g/L and dye conc of 2 ppm. (4) statistical optimization of bacterial removal via CCD to model interactions between adsorbent dose (0–1.18 g/L) and contact time (20–360 min). Results demonstrated that acetone extracts exhibited the highest antibacterial activity (inhibition zones: 9–22 mm), while dry PP achieved 48% MO removal at optimal conditions (1 g/L, 240 min), attributed to polyphenol-mediated adsorption and bacterial membrane disruption. The integrated approach highlights PP’s potential for simultaneous pollutant removal and disinfection in wastewater treatment, offering a sustainable alternative to chemical methods.
Keywords
bioremediation; methyl orange; pomegranate peel; antibacterial; S. aureus
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