Effect of Culture Media on Micropropagation of Pyracantha Fortuneana Plant by Tissue Culture . | ||||
| Horticulture Research Journal | ||||
| Volume 3, Issue 5, June 2025, Page 73-83 PDF (843.3 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/hrj.2025.449607 | ||||
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| Authors | ||||
| Heba, B. Soliman; M.A. El-Shamy | ||||
| Botanical Gardens Res. Dept., Hort. Res. Inst. Agric. Res. Center, Giza, Egypt. | ||||
| Abstract | ||||
| The experimental trial was conducted in the Plant Tissue Culture Laboratory, throughout two years (2022 – 2023). It was intended to find out the well-defined protocol easily for in vitro propagation of Pyracantha fortuneana Roem. In this respect, buds of the plant were surface sterilized with 1.5 % sodium hypochlorite for 20 min. Seeds were sterilized with a mixture of 1.5 % sodium hypochlorite for 30 min. For establishment stage, MS medium at full strength without activated charcoal gave the highest values of shoot length and number of leaves for buds. While, MS full-strength medium with 0.5 mg/l IBA was the best medium for seeds. For multiplication stage, BA at 1.0 mg/l and 1.0 mg/l Kin formed the highest number of shoots for buds and seeds. For in vitro rooting, the highest number of roots/cluster was found at 1.0 mg/l IBA and 0.5 mg/l Kin for buds or 3.0 mg/l IBA and 1.0 mg/l Kin for seeds. The highest percentage of plantlets acclimatized in plastic pots filled with peatmoss alone under greenhouse conditions for Pyracantha buds or seeds. | ||||
| Keywords | ||||
| Pyracantha; Micropropagation; IBA; WPM; CHU | ||||
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