Dissemination of integrons class 1, 2 and 3 among P. aeruginosa isolated from burn cases | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 26 August 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.403582.3018 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Yasser Rabee Ibraheem  1; Majida Malik Alshammari2
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| 1Jabir Ibn Hayyan University for medical and pharmaceutical sciences, faculty of medicine, Iraq. | ||||
| 2Jabir Ibn Hayyan University for medical and pharmaceutical sciences, faculty of medicine, Iraq | ||||
| Abstract | ||||
| Background: Pseudomonas aeruginosa is a principal opportunistic pathogen, frequently responsible for healthcare-associated infections in patients with compromised conditions, such as severe burns. Objective: Evaluation the resistance of antibiotics and examine the presence of Intl I, Intl II and Intl III genes expressing integron classes (CL1, CL2, and CL3) respectively, by PCR and biotyping of integrons positive isolates using ERIC-PCR. Methods: 175 swabs were collected from burn unit (110 males and 65 females) in Baquba Teaching Hospital in Diyala province, Iraq, from September 2024 to January 2025. Isolation and identification by morphological, microscopical and biochemical parameters and diagnosis confirmed by VITEK2 system. Antibiotic susceptibility tests MIC were performed on 70 P. aeruginosa isolates using VITEK2 system AST-GN card. Also, the existence of integron classes and were investigated by PCR assay. Then, Molecular Fingerprinting of isolates done by ERIC–PCR Typing. Results: Results revealed that 70/175 (40%) of bacterial isolates were P. aeruginosa, while 60% were other types of bacteria. 45/70 (64%) of isolates were from males while only 25/70 (35.7%) were recovered from females. Results showed that 95% of isolates were resistance to Kanamycin and Meropenem, 85% for Augmentin, Cefepime and Tobramycin was 63% and 38% respectively. Gentamicin resistance was 33% while netilmicin showed 28% resistance. Amikacin resistance was 5% with high significant difference (p value <0.0001). 47 (67%) of isolates were producing extended spectrum beta-lactamase. Results revealed that 45% and 37.5% of isolates harboring intI and intII gene respectively, while 17.5% of isolates have IntIII gene with high significant difference (P value<0.0001). Moreover, 38 (80%) of P. aeruginosa isolates had ERIC varialble gene. Dendrogram of typable P. aeruginosa isolates produced from ERIC analysis were generated with arithmetic averages (UPGMA). Conclusions: There was high incidence of int1, int2 with the existence of ESBL-producing strains, suggests a significant resistance towards crucial antibiotics of treating of these bacterial infections and underline the significance of antibiotic resistance monitoring in clinical environments. | ||||
| Keywords | ||||
| P. aeruginosa; ESBL; int1; int2 | ||||
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