Quantitative Analysis of FimH Gene Expression in Enterobacter cloacae following Treatment with Silver Nanoparticles | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Volume 35, Issue 2, April 2026 | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2025.413369.1830 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sinaa Kh. Ibrahim  ; Saba J. Jawad Al-Zubaidi
| ||||
| Biology Department, College for Pure Science, Diyala University, Iraq | ||||
| Abstract | ||||
| Background: One of the factors contributing to increase the resistance of bacteria to antibiotic is the presence of virulence factor such as biofilm, fimbriae and efflux pumps. FimH, FimA are the most extensively researched fimbriae genes in Enterobacter cloacae in relation to antibiotic resistance. Objectives: The aim of this study are Isolation and Identification of E.cloacae from patient with UTI, wound, burn infection by using biochemical test and Vitek-2 system then detection the antibiotic resistance and phenotyping biofilm, then gene expression of FimH gene in the presence of study by Sub-minimum inhibitory concentration of sliver nanoparticles. Methodology: A total of 300 clinical specimens were collected from two Diyala Hospitals. The MICs of AgNPs against isolated bacteria was estimated with the microdilution broth method. While the gene expression was done by real-time PCR. Results: The results demonstrated that Enterobacter cloacae were found in 19 of all samples. The minimum Inhibitory Concentration (MICs) of AgNPs at the rang 16-32 μg/ml. Relative Quantification was used to calculate the fold change in gene expression using the delta delta Ct value and the gene expression of the isolates was calculated before and after treated with MIC of each isolate, In FimH gene, by using the sub-MIC values of AgNPs in Enterobacter cloacae resistant isolates before treatment with AgNPs, the fold of gene expression (EC 1,2,3,4 ) was (1) for all the four isolates, and the fold of the resistant isolates after AgNPs treatment was low (0.64, 0.54, 0.00, 0.01) respectively. Conclusion: the obvious activity of AgNPs inhibitor in E. cloacae may be contributed in the management and control the multidrug resistance especially in life threatening infections. | ||||
| Keywords | ||||
| Gene Expression; Enterobacter cloacae; RT-PCR; FimH; AgNPs | ||||
|
Statistics Article View: 4 |
||||
