Rapid, Sensitive, New and Simple UPLC-MS/MS Validated Method with Charged Surface Hybrid Column for Determination of Anti depression Drug Sertraline in Human Plasma and its Application to Bioequivalence Study
Elshayep, Y., Salem, W., Ismail, A., Abd allah, F. (2025). Rapid, Sensitive, New and Simple UPLC-MS/MS Validated Method with Charged Surface Hybrid Column for Determination of Anti depression Drug Sertraline in Human Plasma and its Application to Bioequivalence Study. EKB Journal Management System, (), -. doi: 10.21608/bfsa.2025.401373.2627
Yasser Abd El Fattah Elshayep; waheed Mohamed Salem; Abdelhameed Abdo Ismail; Fathy Ibrahim Abd allah. "Rapid, Sensitive, New and Simple UPLC-MS/MS Validated Method with Charged Surface Hybrid Column for Determination of Anti depression Drug Sertraline in Human Plasma and its Application to Bioequivalence Study". EKB Journal Management System, , , 2025, -. doi: 10.21608/bfsa.2025.401373.2627
Elshayep, Y., Salem, W., Ismail, A., Abd allah, F. (2025). 'Rapid, Sensitive, New and Simple UPLC-MS/MS Validated Method with Charged Surface Hybrid Column for Determination of Anti depression Drug Sertraline in Human Plasma and its Application to Bioequivalence Study', EKB Journal Management System, (), pp. -. doi: 10.21608/bfsa.2025.401373.2627
Elshayep, Y., Salem, W., Ismail, A., Abd allah, F. Rapid, Sensitive, New and Simple UPLC-MS/MS Validated Method with Charged Surface Hybrid Column for Determination of Anti depression Drug Sertraline in Human Plasma and its Application to Bioequivalence Study. EKB Journal Management System, 2025; (): -. doi: 10.21608/bfsa.2025.401373.2627

Rapid, Sensitive, New and Simple UPLC-MS/MS Validated Method with Charged Surface Hybrid Column for Determination of Anti depression Drug Sertraline in Human Plasma and its Application to Bioequivalence Study

Bulletin of Pharmaceutical Sciences Assiut University
Articles in Press, Accepted Manuscript, Available Online from 27 August 2025  XML
Document Type: Original Article
DOI: 10.21608/bfsa.2025.401373.2627
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Authors
Yasser Abd El Fattah Elshayep email 1; waheed Mohamed Salem2; Abdelhameed Abdo Ismail3; Fathy Ibrahim Abd allah4
1Department of chemistry, Faculty of Science ,Menoufia university,ICBR
2Faculty of Applied Health Science Technology, Menoufia University, Shebin Elkom, Egypt
3Faculty of Science, Menoufia University, Shebin Elkom, Egypt
4Faculty of Pharmacy, Department of Pharmaceutics and Industrial Pharmacy, Al- Azhar University, Cairo, Egypt
Abstract
Background: A rapid, simple, sensitive, reproducible, and RP-UPLC-MS/MS method was established and bioanalytically validated for determining Anti depression drug sertraline in human plasma.

Methods: Sertraline was extracted from human plasma by protein precipitation technique using cooled acetonitrile. Sertraline was separated on a C18-column of Acquity UPLC CSH adjusted at 30oC and mass analyzed in a positive electrospray ionization mode. A simple mobile phase of formic acid (0.1%) and acetonitrile in a 50:50 (v/v) ratio was utilized at a flow rate of 300 L/min. the analysis of sertraline was carried out within 2 min over a range 0.5-50.0 ng/mL (r2=0.997).

Results: Accuracy ranged from 95.0 to 104.0%, whereas inter- and intra-precision was not more than 15%. The results confirmed the sertraline stability under various conditions during analysis .

Conclusion: The developed and bioanalytically validated method was effectively applied to a bioequivalence study of sertraline tablet (100 mg) in healthy Egyptian volunteers (n=31).
Keywords
Sertraline; anti-depressants; LC MS/MS; Human plasma; Bioanalytical
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