Identification of Pathogenic Bacteria from Grouper (Epinephelus sp.) Based on 16s rRNA Genes | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 230, Volume 29, Issue 4, July and August 2025, Page 3213-3222 PDF (376.47 K) | ||||
| DOI: 10.21608/ejabf.2025.449766 | ||||
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| Author | ||||
| Afriani et al. | ||||
| Abstract | ||||
| Pathogenic bacteria are microorganisms capable of causing disease in animals and humans, including fish. This study aimed to identify pathogenic bacteria isolated from grouper fish using the 16S rRNA gene. Samples were collected from fish markets in the Central Tapanuli area, with bacterial isolates obtained from the liver, muscle, and stomach tissues. Isolation was carried out using TSA (Tryptone Soya Agar) and BHIA (Brain Heart Infusion Agar) media. Molecular identification of bacterial isolates was performed using the Polymerase Chain Reaction (PCR) method. DNA from selected isolates was extracted using the boiling method, followed by amplification of the 16S rRNA gene. PCR products from six samples yielded fragments ranging from 648 to 839 bp. BLAST analysis of the sequences revealed three types of pathogenic bacteria: Aeromonas hydrophila, Staphylococcus aureus, and Staphylococcus sciuri, with sequence similarity levels of 98.34–100% compared to reference data in GenBank. Phylogenetic analysis using the Kimura 2-Parameter (K2P) model showed that the genetic distance between the study samples and reference sequences in GenBank was less than 2%. This confirms that the isolates obtained were A. hydrophila, S. aureus, and S. sciuri. In conclusion, the 16S rRNA gene proved to be a highly effective molecular marker for the identification of pathogenic bacteria in grouper fish. | ||||
| Keywords | ||||
| Grouper; Aeromonas hydrophila; Staphylococcus aureus; Staphylococcus sciuri; 16s rRNA | ||||
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