The Immune Enchaining Role of Dietary Sulforaphane on the Major Histocompatibility Complex BF-2 (MHC BF-2) Gene Expression as Feed Additives on Broilers
M. Suleyman, M., M. Elbakry, M. (2025). The Immune Enchaining Role of Dietary Sulforaphane on the Major Histocompatibility Complex BF-2 (MHC BF-2) Gene Expression as Feed Additives on Broilers. EKB Journal Management System, 46(3), 661-667. doi: 10.21608/asejaiqjsae.2025.449769
Mahmoud M. Suleyman; Moustafa M. Elbakry. "The Immune Enchaining Role of Dietary Sulforaphane on the Major Histocompatibility Complex BF-2 (MHC BF-2) Gene Expression as Feed Additives on Broilers". EKB Journal Management System, 46, 3, 2025, 661-667. doi: 10.21608/asejaiqjsae.2025.449769
M. Suleyman, M., M. Elbakry, M. (2025). 'The Immune Enchaining Role of Dietary Sulforaphane on the Major Histocompatibility Complex BF-2 (MHC BF-2) Gene Expression as Feed Additives on Broilers', EKB Journal Management System, 46(3), pp. 661-667. doi: 10.21608/asejaiqjsae.2025.449769
M. Suleyman, M., M. Elbakry, M. The Immune Enchaining Role of Dietary Sulforaphane on the Major Histocompatibility Complex BF-2 (MHC BF-2) Gene Expression as Feed Additives on Broilers. EKB Journal Management System, 2025; 46(3): 661-667. doi: 10.21608/asejaiqjsae.2025.449769

The Immune Enchaining Role of Dietary Sulforaphane on the Major Histocompatibility Complex BF-2 (MHC BF-2) Gene Expression as Feed Additives on Broilers

Alexandria Science Exchange Journal
Article 8, Volume 46, Issue 3, September 2025, Page 661-667  XML PDF (296.11 K)
Document Type: Original Article
DOI: 10.21608/asejaiqjsae.2025.449769
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Authors
Mahmoud M. Suleyman email orcid 1; Moustafa M. Elbakry* 2
1Research at the Regional Center for Food and Feed, Agricultural Research Center, Ministry of Agriculture, Egypt.
2Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Abstract
This study investigated the immune-enhancing effects of dietary sulforaphane (SFN) on the Major Histocompatibility Complex BF-2 (MHC BF-2) gene expression in broiler chickens exposed to Aflatoxin B1 (AFB1), a known immunosuppressive mycotoxin. One hundred one-day-old Cobb 500 broilers were divided into five groups: a negative control (no AFB1 or SFN), a positive control (AFB1 only), and three SFN-treated groups (10, 20, and 30 mg/kg SFN with 1 mg/kg AFB1). At day 42, blood samples were analyzed for immunoglobulin levels (IgA, IgY) and BF-2 gene expression using quantitative real time PCR (qPCR). Results showed that AFB1 significantly suppressed BF-2 expression and reduced IgA and IgY levels (p < 0.01). SFN supplementation reversed these effects dose-dependently, with the 20 mg/kg SFN group exhibiting the highest immune enhancement, 1.8-fold increase in BF-2 expression and elevated immunoglobulins (p < 0.01). RNA integrity was preserved in the SFN-treated groups, particularly at a dose of 20 mg/kg. Strong correlations were observed between BF-2 expression, humoral immunity, and CD8+ T-cell counts. These findings suggest that SFN, especially at 20 mg/kg, mitigates AFB1-induced immunosuppression by enhancing immune gene expression, likely via Nrf2/NF-κB pathways, positioning it as a promising natural feed additive for poultry.
Keywords
Sulforaphane; Broilers, Aflatoxin B1 (AFB1); BF-2 gene expression; Immune modulation; MHC class I; qPCR
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