Expression of miR-15a-5p, miR-17-5p, and miR-103-3p in hypertension-associated chronic kidney disease and hemodialysis patients | ||
| Egyptian Pharmaceutical Journal | ||
| Volume 24, Issue 4, October 2025, Pages 62-74 PDF (1.44 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/epj.2025.356567.1030 | ||
| Authors | ||
| Heba Nazeir1; Amr E. Ahmed1; Nahla S. Kotb2; Doaa Ezzat3; Mohamed Gamal4; Mohamed D. E. Abdelmaksoud5; Weaam Gouda* 5 | ||
| 1Department of Biotechnology and Life Science, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt. | ||
| 2Biochemistry Department, Egyptian Drug Authority, Giza, Egypt. | ||
| 3Medical Biochemistry and Molecular Biology, College of Oral and Dental Surgery, Misr University for Science and Technology, Egypt. | ||
| 4Internal Medicine Gastroenterology and Hepatology, Faculty of Medicine, Ain Shams University, Cairo, Egypt. | ||
| 5Biochemistry Department, Biotechnology Research Institute, National Research Centre, Giza, Egypt. | ||
| Abstract | ||
| Background MicroRNAs (miRs), which can be released into the extracellular environment, are important modulators of how cells react to a variety of stimuli. Prior research in the past ten years has concentrated on miRs as new clinical targets or diagnostic tools. Numerous studies on chronic kidney disease (CKD) have focused on miRs. One prevalent risk factor for CKD is hypertension (HTN), which damages targeted organs, including the kidneys, heart, and arteries. Objective Our goal was to investigate the expressions of miRs (17-5p, 15a-5p, and 103-3p) in prerenal failure and renal failure patients under hypertension conditions, as these could be biomarkers of kidney damage caused by hypertension. Patients and methods Quantitative real-time polymerase chain reaction (RT-PCR) was utilized to assess the expression levels of the above-mentioned miRs. A total of 110 studied cases that met the eligibility criteria of HTN-associated CKD were grouped into a pre-renal failure group (n = 50) and a renal failure group (n = 60), which included end-stage renal disease cases under hemodialysis beside 40 healthy controls. Results and conclusion Our research revealed that miR-103-3p was upregulated; however, miRs (15a-5p and 17-5p) were significantly downregulated in studied HTN-CKD groups compared with control subjects. The degree of deregulation correlated with the severity of the disease suggests that these miRs may be involved in the pathophysiology of HTN-related CKD, which in turn encourages additional research into their possible clinical use in the prevention and management of CKD. Moreover, these miRs showed areas under the curve near to 1 in receiver operating characteristics (ROC) analysis. Consequently, they might be used as markers for CKD. | ||
| Keywords | ||
| Keywords: chronic kidney disease (CKD); hypertension (HTN); microRNAs (miRs); miR-103-3p; miR-15a-5p; miR-17-5p | ||
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