Spa technique for typing of Staphylococcus aureus isolated from patients with health care associated infection in Tanta University Hospitals

Eid, Amira Ezzat Zaki; Abo Elnasr, Lobna Mohamed; Hassan, Aziza Mahmoud

doi:10.21608/mid.2025.411719.3088

	Spa technique for typing of Staphylococcus aureus isolated from patients with health care associated infection in Tanta University Hospitals
Microbes and Infectious Diseases
Articles in Press, Accepted Manuscript, Available Online from 31 August 2025
Document Type: Original Article
DOI: 10.21608/mid.2025.411719.3088
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Authors
Amira Ezzat Zaki Eid ¹; Lobna Mohamed Abo Elnasr²; Aziza Mahmoud Hassan¹
¹Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta university, Egypt
²Anaesthesia and Surgical Intensive Care department, Faculty of Medicine, Tanta University, Egypt
Abstract
Background: Staphylococcus aureus is an important cause of health care associated infection (HCAI) it has a broad spread in both community and hospital settings. Its importance increased a with the marked development of drug resistance among its isolates to various antibiotic particularly methicillin (MRSA) so it is important to detect various strains and identify its source for proper management . There are numerous methods for typing of S. aureus. Spa typing is effective ,relatively in expensive with good reproduciblity. Objectives : To assess the the spa gene diversity among S. aureus isolates, genotype clinical S. aureus isolates in Tanta University Hospitals and the patterns of antibiotic sensitivity & resistance among various isolates. Methods: one hundred samples were gathered under complete aseptic measures and underwent the following: standard microbiological techniques for detection of staphylococcal isolates then antibiotic susceptibility and Spa typing is performed and DNA sequencing and analysis by Chromas software. Results: S.aureus was the most prevalent isolate (65%) ,the prevalence of MRSA was 69.2 %. 49 (75.4%) of the 65 strains displayed Spa gene . Six distinct spa types were identified. t304 (15 isolates, 30.6%), t307 (10 isolates, 20.4 %), t037 (9 isolates ,18.3 %), t030 (8 isolates ,16.3 %), t346 (5 isolates, 10.2%), t044 (2 isolates, 4.1%).Conclusions: S.aureus is a consequential cause of HCAI. MRSA has a wide spread. Spa typing is a good ,relatively inexpensive and reproducible technique for typing of S.aureus.
Keywords
HCAI; MRSA; Spa types; Chromas software


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