Detection of the antioxidant activity of fungi and bacteria-derived secondary metabolites

Al-doori, Ameerah Abdulmunem Yaseen; Hasan, Shelan Weli; Yaseen, Israa Abdel Munem Yaseen Abdel Munem; Darwish, Shaima Ahmed Hussein; Alabbasi, Aqeel Majid Alabbasi; Dheeb, Batol Imran

doi:10.21608/ijabmb.2025.411351.1019

	Detection of the antioxidant activity of fungi and bacteria-derived secondary metabolites
International Journal of Applied Biochemistry and Molecular Biology
Volume 2, Issue 2, June 2025, Page 142-161 PDF (378.29 K)
Document Type: Review Article
DOI: 10.21608/ijabmb.2025.411351.1019
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Authors
Ameerah Abdulmunem Yaseen Al-doori¹; Shelan Weli Hasan²; Israa Abdel Munem Yaseen Abdel Munem Yaseen³; Shaima Ahmed Hussein Darwish⁴; Aqeel Majid Alabbasi Alabbasi³; Batol Imran Dheeb ²
¹University of Samarra/College of Applied Sciences/Department of Applied Chemistry, Samarra, Iraq
²Samara University, Faculty of Applied Sciences, Department of Pathological Analysis, Samara, Iraq
³University of Samarra/College of Education /Department of Biology, Samarra, Iraq
⁴Al-Karkh University of Science, Baghdad, Iraq
Abstract
Fungal and bacterial metabolites were used to create a pleasant, appropriate method, which was a prerequisite for scientists to produce natural compounds with strong biological activity from inexpensive sources and to recover the average of antiproliferative agents. Thin-layer chromatography was used to identify fungal metabolites in the current findings. The most powerful antioxidant, antiproliferative, and antibacterial activity was found in one fungal isolate out of ten that underwent bioassay; this isolate was determined to be Aspergillus flavus. With a low IC50 concentration and a strong flavonoid content, the fungus extract of A. flavus demonstrated a significant percentage of free radical scavenging activity utilizing Diphenyl-1-picrylhydrazyl (DPPH). The presence of several strong products of A. flavus extract is demonstrated by the results of an instrumental analysis performed using GC.Mass. Furthermore, the extract from A. flavus exhibited encouraging antiproliferative properties. Under these experimental circumstances, inhibitory efficacy against Hepatocellular Carcinoma Cells was found. It is also possible to utilize this approach to extract modern medications.
Keywords
Antimicrobial endophyte; secondary metabolites; TPC; TFC; oxidative stress


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