OPTIMIZATION THE COMPOSITION OF THE MICROBIAL GROWTH MEDIUM FOR THE PRODUCTION OF Β- GALACTOSIDASE BY YEASTS | ||||
| Egyptian Journal of Applied Science | ||||
| Volume 40, Issue 7, July 2025, Page 39-54 PDF (869.69 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejas.2025.450712 | ||||
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| Abstract | ||||
| ABSTRACT The study was undertaken to evaluated the effect of some carbon and nitrogen sources as well as some cheap and locally agricultural or industrial wastes i.e., sugar cane bagasse and permeate on β- galactosidase production by Kluyveromyces lactis, No.(1) and Pichia kudriavzevii No.(13). Results revealed that, in general galactose was the best carbon source followed by lactose, maltose and sucrose, for both two tested strains. The media containing galactose at concentration of 13% gave the highest enzymatic activity being 801.57 and 769.48 (nmol/ml/min.) for Kluyveromyces lactis, No.(1) and Pichia kudriavzevii No.(13), respectively. In addition, results indicated that, in general, yeast extract was the best nitrogen source followed by peptone and malt extract, for the both two tested strains. A concentration of 0.5 % gave the highest enzyme yield for Kluyveromyces lactis No. (1), while at 1.5% for Pichia Kudriavzevii No. (13). Also, results indicated that the maximum values of enzyme production were in the control medium which containing 0.1% NH4H2PO4 as well as (NH4)2HPO4 for the both investigated yeast strains. Also, results indicated that, 7.0 % concentration of sugar cane bagasse was the most suitable level of enzyme production (317.86 and 351.24 nmol/ml/min.) being about 39.25% and 44.35% of that produced in the control medium for Kluyveromyces lactis No.(1) and Pichia Kudriavzevii No.(13), respectively. Concerning, the permeate medium, the concentration of 70% gave the highest activity of enzymem was about 82.18 % and 81.63% of the control medium for Kluyveromyces lactis No.(1) and Pichia Kudriavzevii No.(13) respectively. | ||||
| Keywords | ||||
| carbon and nitrogen sources; agro-Industrial wastes | ||||
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