Intra-Articular Injection of Human Placental Extract for Management of Temporomandibular Joint Osteoarthritis in Male Wistar Rats: An Experimental Study | ||||
| Dental Science Updates | ||||
| Volume 6, Issue 2, September 2025, Page 329-339 PDF (1.62 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/dsu.2025.355045.1283 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ahmed Sayed Ahmed Eltaweel1; Mohamed Said Hamed1; Mona K Tawfik 2; Mohamed Kamal El-kherbetawy3; Ahmed Abdelmohsen Younis Ahmed Ali  4
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| 1Oral and Maxillofacial surgery, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt. | ||||
| 2Pharmacology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
| 3Pathology Faculty of Medicine Suez Canal University, Ismailia, Egypt. | ||||
| 4oral and Maxillofacial Surgery, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt | ||||
| Abstract | ||||
| Introduction: Temporomandibular Joint Osteoarthritis (TMJ OA) is a type of osteoarthritis that specifically affects the temporomandibular joint (TMJ). Human placental extract (HPE) has shown potential as a therapeutic option for treating temporomandibular joint osteoarthritis (TMJ OA). Aim: To evaluate the regenerative effect of HPE in the management of degenerative TMJ OA in male rats. Methods: This investigation conducted on 32 male rats, divided into four groups of eight animals each; Group I, served as a control, receiving only saline injections and thus representing a baseline, untreated state. Group II, the temporomandibular joint (TMJ OA) control group, was subjected to a surgical procedure that created a full-thickness osteochondral defect via power drills, and these animals were subsequently terminated two weeks post-procedure. Group III was subjected to the same surgical procedure as group II then administered HPE via intra-articular injections into the TMJ at a dosage of 10 mg/kg daily for a duration of two weeks; these rats were then euthanized in the fourth week. Group IV was subjected to the same surgical procedure as group II then administered HPE via intra-articular injections into the TMJ at a dosage of 10 mg/kg daily for a duration of two weeks; these rats were then euthanized in the fifth week. Results: Histological evaluation of samples of all rats which injected intra-articular injection of HPE on TMJ, proved that HPE facilitated healing of temporal bone and articular disc in comparison with other groups, group IV HPE treated group with sacrificing at 6th week was the nearest to normal group I. Blood results revealed that all rats which injected intra-articular of HPE on TMJ showed a reduction in levels of inflammatory markers IL-1β, IL-6, and CRP. Conclusion: HPE has anti-inflammatory and regenerative properties on TMJ OA according to histological evaluations of rats and blood sample evaluations by reducing inflammatory markers such as IL-1β, IL-6, and CRP. | ||||
| Keywords | ||||
| Histopathological Examination; Human Placental Extract; Temporomandibular Joint Osteoarthritis | ||||
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