Comparative Immunohistochemical Assessment of the Effects of Chitosan and Curcumin on dorsal surface of tongue mucosa of albino rats fed on high-fat diet:Histological and Immunohistochemical Study | ||
| Dental Science Updates | ||
| Volume 6, Issue 2, September 2025, Pages 355-367 PDF (3.31 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/dsu.2025.362383.1290 | ||
| Authors | ||
| Amr Ahmed El-Sayed* 1; Elham fathi mahmoud2; Rasha Mohamed Taha3 | ||
| 1Oral Biology department, Faculty of Dentistry, Suez Canal university, Cairo, Egypt | ||
| 2head of oral biology department, Faculty of Dentistry, Suez Canal University | ||
| 3oral biology department, Faculty of Dentistry, Suez Canal University | ||
| Abstract | ||
| Introduction: High-fat diet consumption results in raised levels of cholesterol in the blood with a massive effect on tongue mucosa, according to previous research. Recently it was claimed that using natural products such as curcumin and chitosan has the potential to decrease cholesterol levels, which positively affects the dorsal surface of the tongue. Aim: This work purposed to assist the effects of curcumin and chitosan as a natural product on the dorsal surface of tongue mucosa of albino rats fed on a high-fat diet. Materials and Methods: 40 rats were divided into 2 main groups (1 – control group: received normal diet and distilled water, 2 – experimental groups: divided into 3 subgroups: 2-A received High fat diet and Cholesterol powder (10gr), 2-B curcumin treated group at the beginning of the fourth month with 1.5 g curcumin/ kg powder dissolved in distilled water, and 2-C chitosan treated group at the beginning of the fourth month with 500 mg/ Kg. BW Chitosan tab which added to a distilled water in a concentration of 10% (curcumin and chitosan were administered to rats via gastric tube). Cholesterol level in blood was measured at days 0, 90 and 120. Rats were euthanised at the end of 4 months, and tongue specimens were processed for histological and immunohistochemical evaluation. All data were statistically analysed using a one-way ANOVA (F) test with a post hoc test (Tukey). Results: A high-fat diet caused degeneration to the tongue’s dorsal surface; treatment with curcumin and chitosan showed a better histological effect compared with high fat diet group. Chitosan showed near-normal papillae structure with partial muscle regeneration. Conclusions: A high-fat diet induces cytotoxic effects on tongue mucosa, while curcumin and chitosan demonstrate protective potential. | ||
| Keywords | ||
| High fat diet; Curcumin; Chitosan; Tongue | ||
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