Effects of Omega 3 Polyunsaturated Fatty Acid against Acrylamide-Induced Toxicity in Submandibular Salivary Glands of Albino Rats: A Histological and Molecular Study | ||||
| Dental Science Updates | ||||
| Volume 6, Issue 2, September 2025, Page 395-402 PDF (1.78 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/dsu.2025.370807.1296 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Salma Awad Taghyan   1; Elham Fathy Mohamed2; Rasha Taha 2; Mohamed Shamel3
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| 1Department of Oral Biology, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt | ||||
| 2Department of Oral Biology, Faculty of Dentistry, Suez Canal University | ||||
| 3Department of Oral Biology, Faculty of Dentistry, The British University in Egypt | ||||
| Abstract | ||||
| Introduction: Acrylamide (AA), a water-soluble compound with high chemical activity that can be found in widely consumed food products. Aim: To evaluate the protective effect of Omega 3 polyunsaturated fatty acid (ω3-PUFAs) against AA induced toxicity on the submandibular salivary glands (SMGs) of Albino rats. Materials and methods: Thirty male albino rats weighing 150 – 200 gm were equally and randomly divided into control group, which received normal saline vehicle daily via oral gavage for 30 days, AA group received 15 mg/kg body weight (bw) of AA dissolved in 0.2 ml saline solution daily via oral gavage for 30 days. ω3-PUFAs group received 15 mg/kg bw of AA combined with 0.4 g/kg of ω3-PUFAs daily via oral gavage for 30 days. The rats were euthanized, and SMGs were dissected for histological evaluation, including hematoxylin and eosin staining (H&E) and immunohistochemistry for tumor necrosis factor (TNF-α), as well as analysis for heme-oxygenase-1 gene (HO-1) expression using real-time Polymerase chain reaction (RT-qPCR). Results: The SMG of AA group showed signs of toxicity and degeneration in the form of ill-defined outlines with different-sized cytoplasmic vacuolations, pyknotic and crescent-shaped nuclei that were statistically significant, with an increase in TNF-α immunoexpression and HO-1 gene expression. ω3-PUFAs administration mitigated the toxic effect following AA exposure and down-regulated the TNF-α and HO-1 gene expression. Conclusion: The study revealed a significant cytotoxic effect of AA on SMGs of albino rats, presumably by generation of oxidative stresses and mitochondrial dysfunction. ω3-PUFAs effectively alleviated these toxic effects, indicating its antioxidant potential. | ||||
| Keywords | ||||
| Salivary glands; Acrylamide; Omega 3; Oxidative stress | ||||
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