Pro-inflammatory Cytokines of Gastric Tissues and Its Role in Patients with H. pylori | ||||
| Microbial Biosystems | ||||
| Article 26, Volume 10, Issue 3, September 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mb.2025.349338.1223 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Batool M Kareem Mohammed  1; Maysaa Salih2; Ali Gaber3
| ||||
| 1Department of Medical Microbiology/University of Babylon, Babylon, Iraq | ||||
| 2*Department of Medical Microbiology/University of Babylon, Babylon, Iraq | ||||
| 3Department of Internal Medicine/University of Babylon, Babylon, Iraq | ||||
| Abstract | ||||
| Helicobacter pylori infection, affecting over 50% of the global population, is a major cause of peptic ulcer disease due to its robust induction of pro-inflammatory cytokines like Tumor Necrosis Factor-alpha1 and Interleukin-1 Beta. These cytokines recruit immune cells, leading to tissue damage and ulceration. Understanding the dysregulated cytokine cascade is crucial for developing effective therapeutic strategies. The aim of the study is to investigate the role of pro-inflammatory cytokines Tumor Necrosis Factor-alpha1 and Interleukin-1 Beta in the pathogenesis of Helicobacter pylori -induced peptic ulcers by quantifying their concentrations in mucous layer of gastric biopsy samples. A total of 65 patients presenting with epigastric pain and gastroenterologist had been diagnosed as peptic ulcer and 25 healthy controls have been involved in the study. Gastric biopsies were obtained to detect of Helicobacter .pylori by using quantitative real-time PCR (qRT-PCR) to measure copy numbers (rpoD gene). The concentration of Interleukin-1 Beta and Tumor Necrosis Factor-alpha1 in biopsy suspension (mucus layer) samples were determined using an ELISA assay (Solarbio Human IL-1β ELISA Kit), and absorbance was measured with a Fisher Scientific ELISA plate reader. Patients with peptic ulcers and positive to Helicobacter. pylori demonstrated significantly elevated concentrations of both IL-1β (17.78 pg/ml vs. 9.05 pg/ml) and TNFα (89.84pg/ml vs. 34.67pg/ml) compared to controls, with both differences reaching statistical significance (p<0.001). The study highlights a significant elevation of TNFα and IL-1β in Helicobacter. pylori-induced peptic ulcer patients, indicating their critical role in inflammation and ulcer pathogenesis. | ||||
| Keywords | ||||
| IL-1β; TNFα; Peptic Ulcer; Helicobacter. pylori; Cytokine | ||||
|
Statistics Article View: 6 |
||||
