A New Trial to Induce Metabolic Disorders in Rats by Dietary Egg Yolk Supplementation with High-Fat Diet: A Comprehensive Study on BloodProfile and Selected Biochemical and Hormonal Parameters | ||
| Egyptian Academic Journal of Biological Sciences, B. Zoology | ||
| Volume 17, Issue 2, December 2025, Pages 81-103 PDF (813.49 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsz.2025.451990 | ||
| Authors | ||
| Rawda E. Alnfrawy1; Ahkam M. El- Gendy1; Salah M. Alshahat2 | ||
| 1Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt. | ||
| 2Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Boys branch), Cairo, Egypt. | ||
| Abstract | ||
| This study aimed to establish a novel model for inducing metabolic disorders in male albino rats through the incorporation of egg yolk supplements, in raw and cooked forms, alongside a high-fat diet (HFD). Over two experimental periods 6 and 12 weeks—the morphological, hematological, biochemical, and hormonal impacts of these dietary interventions were assessed. Experimental groups included rats fed HFD alone or in combination with either low or high doses of raw by gastric tube or boiled egg yolk by added to their diet, as well as a group receiving doses of yolk with a normal diet. Key parameters included body weight progression, liver and internal fat relative weight, complete blood count (CBC), glucose homeostasis markers (FBS, insulin, HbA1c, HOMA-IR), lipid profile (TGs, cholesterol fractions), liver function enzymes, thyroid hormones (TSH, T3, T4), and testosterone hormone. The results demonstrated that HFD and its combination with both low and high-dose raw yolk significantly aggravated obesity indices, insulin resistance and lipid abnormalities, in contrast, yolk supplementation under normal dietary conditions appeared to exert protective or neutral effects on most parameters. These findings suggest that raw egg yolk, particularly in the context of a high-fat diet, can potentiate metabolic disturbances, while its impact is modulated by the dietary background and method of preparation.This experimental model provides valuable insights for future investigations on diet-induced metabolic dysregulation. | ||
| Keywords | ||
| Metabolic disorders; egg yolk; High Fat Diet; vegetable ghee; oxidative stress. | ||
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