Molecular and Serological Identification of Neospora caninum and The Associated Biochemical Changes in Egyptian Dairy Cattle. | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 10 September 2025 PDF (678.55 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.318924.2363 | ||
| Authors | ||
| amal Mahmou Abo El-Maaty* 1; Ashraf Hasan Soror1; El-Sayed Nabil El-Alfy2; Ghada Afifi3; Khaled A. Attwa1; magdy elgioushy mahmoud4 | ||
| 1National Research Centre | ||
| 2Faculty of Veterinary Medicine, Mansoura University, Mansoura | ||
| 3department of Genetic Engineering and Biotechnology Research Institute, Sadat City University, | ||
| 4AswanUniversity | ||
| Abstract | ||
| For detecting N. Caninum in dairy cows and their associated biochemical and inflammatory markers, 77 blood samples were collected from animals were subjected for serological and molecular testing. Myeloperoxidase (MPO), interlukin-10 (IL-10), catalase, nitric oxide (NO), malondialdehyde (MDA), calcium and phosphorus were compared in serologically negative animals with sensitivity/specificity <30 % with those serologically positive chronic (S/P >40% and <100 %), and serological positive acute (S/P>100). Results indicated that 21animals were seropositive (27.27%). From the seropositive animals 10 (47.62%) were considered as chronic having S/P of 63.59±11.49 and 11 (52.38%) were considered acute having S/P of 200.59±71.78. Only, one suspected animal by serological (1.29%) testing was detected positive by PCR. Seropositive chronic cows had high (P>0.05) MPO but seropositive acute case had the highest IL-10 and phosphorus (P<0.01) associated the lowest calcium (P<0.05) and calcium phosphorus ratio. In conclusion, serological test can be used to distinguish between serological acute and chronic positive cases while molecular test can be used to confirm the infection in suspected cases. Additionally, levels of IL-10, calcium, phosphorus, or calcium/phosphorus ratio can act as biomarkers for acute neosporosis in dairy animals. | ||
| Keywords | ||
| Bovine neosporosis; ELISA; PCR; IL-10; inflammatory markers; minerals | ||
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