Isolation and Molecular Identification of Novel PhosphateSolubilizing Bacteria and Their Plant Growth-Promoting Activities. | ||
| Minia Journal of Agricultural Research and Development | ||
| Volume 45, Issue 4, December 2025, Pages 699-715 PDF (702.23 K) | ||
| Document Type: Original Articles | ||
| Abstract | ||
| This investigation aimed to isolate and characterize novel phosphate-solubilizing bacterial (PSB) isolates using both conventional and molecular techniques. Additionally, the study evaluated their plant growth promoting activity on sugar beet (Beta vulgaris). Three distinct colonies that produced clear halos on Pikovskaya's agar (PKA) plates were selected and designated as YMP5, YMP6, and YMP7 for microbiological and molecular characterization. Due to the 16S rRNA gene sequence analysis and comparison with strains in the NCBI GenBank database, isolates YMP5 and YMP7 were identified as Pseudomonas indica, while isolate YMP6 belonged to the genus Priestia. Phylogenetic analysis further confirmed that YMP5 and YMP7 clustered with Pseudomonas indica strains IMT37 and NBRC 103045, whereas YMP6 clustered with Priestia endophytica strain 2DT. The 16S rDNA gene sequences of these PSB isolates were submitted to the NCBI database under accession numbers PV652948 to PV652950. In terms of plant growth promoting activities, all isolates positively influenced vegetative growth parameters, enhanced NPK nutrient content, and increased photosynthetic pigment concentrations compared to the untreated control. These findings suggest that beneficial PSB strains can be isolated from Egyptian soils and utilized as sustainable bio-inoculants in agriculture. Such bio-fertilizers have the potential to enhance plant development, reduce reliance on chemical fertilizers, and mitigate crop yield losses. | ||
| Keywords | ||
| PSB; Pseudomonas; Priestia; 16S rRNA sequence; vegetative growth | ||
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