Molecular detection of Extended Spectrum Beta-Lactamase genes in a Beta-Lactam resistant E. coli isolated from urinary tract infection patients in some selected hospitals in Gusau Metropolis, Nigeria | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 11 September 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.233417.1607 | ||
| Authors | ||
| Jafar Usman* 1; Fatima Sanusi2; Nurudeen Salisu3; Saifullahi Gambo4; Mansur Usman3; Muhammad Usman Damani5; Sufiyanu Saminu6; Ahmad Muhammad Sadisu7; Baraatu Isah Moriki7; Abubakar Abdullahi8; Hussaini Sani8; Shafaatu Ahmad Oiza7; Sadiq Musa9 | ||
| 1Department of Biochemistry, Federal University Gusau, Nigeria. | ||
| 2Department of Nursing, Federal Medical Center, Gusau, Nigeria. | ||
| 3Department of Microbiology, Federal University Gusau, Nigeria. | ||
| 4Department of Microbiology, Federal University, Gusau, Nigeria. | ||
| 5Department of Microbiology, Usmanu Danfodiyo University Sokoto, Nigeria. | ||
| 6Department of Biological Sciences, Federal University Gusau, Zamfara State, Nigeria | ||
| 7Department of Biochemistry and Molecular Biology, Zamfara State University, Talata Mafara, Nigeria. | ||
| 8Department of Biochemistry Federal University Gusau, Nigeria. | ||
| 9Department of Biochemistry, Federal University Birnin Kebbi, Nigeria. | ||
| Abstract | ||
| Background: Urinary tract infections (UTIs) are among the most common bacterial infections worldwide, with Escherichia coli (E. coli) being a predominant causative agent. The emergence of beta-lactam resistance in E. coli, particularly mediated by extended-spectrum beta-lactamase (ESBL) genes, poses a significant public health challenge. This study aimed to investigate ESBL genes in beta-lactam resistant E. coli strains isolated from UTI patients in selected hospitals within Gusau Metropolis, Nigeria. Methods: A total of 92 urine samples were collected from UTI patients attending General Hospital in Gusau Metropolis. Isolation and identification of E. coli strains were performed using standard microbiological techniques. Antimicrobial susceptibility testing was conducted according to CLSI,2023. Molecular detection of ESBL genes TEM, SHV, CTX-M and OXA was performed using polymerase chain reaction (PCR) assays. Results: Out of 96 samples collected only 19 were positive for E. coli, the study revealed the presence of beta-lactam resistance genes among E. coli isolates from UTI patients in Gusau Metropolis, predominantly SHV and TEM (80% each), followed by CTX-M (60%) and OXA (20%). To the best of our knowledge this is the first publication reporting the presence of beta-lactam resistance genes in E. coli isolated in Gusau Zamfara state, Nigeria. Conclusion: This research highlights the alarming spread of ESBL-producing E. coli in UTI patients in Gusau Metropolis and underscores the importance of molecular surveillance in understanding the epidemiology and genetic diversity of ESBL genes. Such information is crucial for guiding infection control measures, optimizing antibiotic therapy, and addressing the growing threat of antibiotic resistance in the region. | ||
| Keywords | ||
| UTIs; ESBL; E. coli; beta-lactam; Prevalence | ||
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