Anti-bacterial activity of genetically characterized Actinomycetes against urogenital infections related to male infertility | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 13 September 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.414608.3122 | ||
| Authors | ||
| Ayat Saied* 1; Eman Anwar Hassan1; Hany Sabry1; Zeinab Khaled Abd El-Aziz2 | ||
| 1International Islamic center for population studies and research Al-Azhar University, Egypt | ||
| 2Botany and Microbiology Department, Faculty of Science (Girls), Al-Azhar University, Nasr City, Cairo, Egypt | ||
| Abstract | ||
| Background: Actinomycetes are scarce in natural product information systems, but their value is frequently underestimated. Although working with these genera presents significant experimental challenges, such as genetic tractability, isolation, and metabolite producer, they have significant biosynthetic potential, and it is valuable to produce strategies to take advantage of these microorganisms. It is important to isolate various actinomycetes from various habitats and test its biological activities especially its antimicrobial impact. In this work we focused on isolation of a Actinomycete strain with effective antimicrobial activity towards previously isolated MDR bacterial stains from semen. Methods: Soil specimen was acquired from El-fayoum vitro and screened from actinomycetes isolates. The isolated actinomycete was grown in starch nitrate broth media to produce extracellular metabolites in the filtrate, which has been used as an antimicrobial agent by agar diffusion assay towards Staphylococcus aureus, Klebsiella pneumoniae, Morganella morganii, and Escherichia coli which were previously isolated from semen. Morphological, biochemical, and chemotaxonomic tests have been used to identify the actinomycete isolate. 16S rRNA was used to confirm the obtained identification. Results: The filtrate of actinomycete exhibited antimicrobial action versus Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Morganella morganii, and the isolate's aerial hyphae were gray, but its substrate mycelia were off-white, and it developed light brown diffusible pigments on oatmeal agar. The isolate molecularly identified as Streptomyces griseus with code of OP782088.1. Conclusion: Streptomyces griseus OP782088.1, exhibits an effective antimicrobial effect against resistant bacteria isolated from semen. Future studies ought to prioritize improving the environmental parameters, such as temperature and nutrient sources, for actinomycetes to produce more efficient antimicrobials. | ||
| Keywords | ||
| Actinomycetes; resistant; antimicrobial; Streptomyces | ||
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