Association of prevalence of type 3 secretion system in Pseudomonas aeruginosa isolates and their antibiotic resistance | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 13 September 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.415558.3130 | ||
| Authors | ||
| Mahmoud E. Habib1, 2; Sarah A. Abdelkader1; Mohammed El-Mowafy* 1 | ||
| 1Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt | ||
| 2Department of Microbiology and Immunology, Faculty of Pharmacy, Horus University, New Damietta, 34517, Egypt | ||
| Abstract | ||
| Background: Pseudomonas aeruginosa is a Gram-negative, opportunistic pathogen with significant clinical implications, especially for immuno-compromised patients. Type III Secretion System (T3SS) in P. aeruginosa was founded to contribute to the pathogenicity of this bacterium due to the secretion of T3SS related effector proteins; ExoT, ExoY, ExoU, and ExoS that are crucial to the virulence of P. aeruginosa. In this work we were interested in investigating whether there is an association between the predominance of T3SS genes (exoT, exoY, exoU, and exoS) and antibiotics resistance in P. aeruginosa. Methods: The antibiotic resistance to different antimicrobials were determined for clinical isolates P. aeruginosa. Moreover, the predominance of T3SS genes in these isolates. Furthermore, associations between the presence of T3SS genes and antibiotic resistance were determined by Fischer exact test. Results: Our results showed that all strains were resistant to ceftazidime and cefepime, while the lowest resistance rates were observed with meropenem (41.37%). Regarding the prevalence of T3SS genes, exoT, exoS, exoU, and exoY were present in 67.2, 58.6, 56.9 and 13.8 % of the isolates respectively. Our study demonstrates that there was a significant association between the presence of exoY gene and resistance to ciprofloxacin and meropenem (at p<0.05) in P. aeruginosa. Moreover, there was a significant association between the presence of exoT gene and resistance to meropenem (at p<0.05). Conclusion: We recommend that further studies should be conducted to understand the mechanism behind such association. | ||
| Keywords | ||
| Pseudomonas aeruginosa; Type III Secretion System; Antimicrobial resistance | ||
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