Antibiofilm Activity of Naproxen against Bacterial Isolates from Sinusitis and Tonsillitis Patients
Abdul-Hussein, Z., Abdul-Samad, A., Khamees, A., Ali, H. (2026). Antibiofilm Activity of Naproxen against Bacterial Isolates from Sinusitis and Tonsillitis Patients. EKB Journal Management System, 35(2), -. doi: 10.21608/ejmm.2025.419440.1849
Zainab R. Abdul-Hussein; Ali T. Abdul-Samad; Ahmed S. Khamees; Hadeel M. Ali. "Antibiofilm Activity of Naproxen against Bacterial Isolates from Sinusitis and Tonsillitis Patients". EKB Journal Management System, 35, 2, 2026, -. doi: 10.21608/ejmm.2025.419440.1849
Abdul-Hussein, Z., Abdul-Samad, A., Khamees, A., Ali, H. (2026). 'Antibiofilm Activity of Naproxen against Bacterial Isolates from Sinusitis and Tonsillitis Patients', EKB Journal Management System, 35(2), pp. -. doi: 10.21608/ejmm.2025.419440.1849
Abdul-Hussein, Z., Abdul-Samad, A., Khamees, A., Ali, H. Antibiofilm Activity of Naproxen against Bacterial Isolates from Sinusitis and Tonsillitis Patients. EKB Journal Management System, 2026; 35(2): -. doi: 10.21608/ejmm.2025.419440.1849

Antibiofilm Activity of Naproxen against Bacterial Isolates from Sinusitis and Tonsillitis Patients

Egyptian Journal of Medical Microbiology
Volume 35, Issue 2, April 2026
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2025.419440.1849
Authors
Zainab R. Abdul-Hussein* 1; Ali T. Abdul-Samad2; Ahmed S. Khamees3; Hadeel M. Ali4
1Department of Pathological analyses, College of Science, University of Basra, Iraq
2Al- Basrah Teaching Hospital, Ministry of Health, Basra, Iraq
3Department of Pathological analyses, College of Science, University of Basra
4Oncology and Blood Diseases Center, Basrah Health Department
Abstract
Background: Biofilm formation is a significant virulence factor in bacterial infections, contributing to antibiotic resistance and chronic infections. Objective: Its aims to investigated the microbial profile of sinusitis and tonsillitis infections and evaluated the antibacterial and antibiofilm effects of naproxen against clinical isolates. Methodology: A total of 126 swab samples (42 sinusitis, 84 tonsillitis) were collected and cultured on selective media, yielding 366 bacterial isolates identified by Vitek 2 system. Predominant species included Staphylococcus aureus (35.5%), Pseudomonas aeruginosa (23.5%), and Streptococcus pyogenes (11.7%), with significant species-specific infection associations (p<0.05). Results: Biofilm formation assessed via Congo Red Agar revealed 59.3% of isolates were biofilm producers (97 strong, 120 moderate). Naproxen demonstrated concentration-dependent antibacterial activity, showing greatest efficacy against Gram-positive cocci (inhibition zones 16-30 mm) but limited activity against P. aeruginosa and P. putida at concentrations ≤1000 μg/mL. Notably, sub-inhibitory naproxen concentrations significantly reduced biofilm formation in treated isolates compared to controls (p<0.01). Conclusion: Naproxen possesses both antimicrobial and biofilm-disrupting properties, supporting its potential therapeutic utility in biofilm-associated respiratory infections.
Keywords
Antimicrobial Activity; Biofilm; Naproxen; Sinusitis; Tonsillitis
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