Molecular Characterization of Pigeon Pea (Cajanus cajan (L.) Millsp.) by RAPD and ISSR Markers | ||
| Egyptian Journal of Agronomy | ||
| Volume 47, Issue 4, December 2025, Pages 835-840 PDF (560.57 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/agro.2025.333401.1555 | ||
| Authors | ||
| Sherin A Mahfouze* 1; negm A Abdel Samea1; Heba Mahfouze2 | ||
| 1National Research Centre | ||
| 2National Research Centre | ||
| Abstract | ||
| Pigeon pea (Cajanus cajan [L.] Millsp.) plant is an important legume crop. The study aimed to molecular characterization of C. cajan [L.] Millsp plant (leaf and seed) by RAPD and ISSR loci as marker-assisted selection (MAS) in the plant breeding programs. DNA extracted from the leaf, five out of ten RAPD primers gave 23 amplicons, an average of 4.6 per primer. Primer RAPD-C1 gave the most number of amplified amplicons (seven). In contrast, primer RAPD-B9 displayed the least number of PCR products (two bands). For seed, the seven out of ten RAPD primers that yielded 26 PCR products ranged from 220 bp (primer RAPD-A13) to 1360 bp (primers RAPD-A5 and RAPD-B4), with a mean of 3.71 per primer. Both primers RAPD-A5 and RAPD-B4 showed the greatest number of amplicons (five). On the contrary, primer RAPD-C17 showed the smallest number of amplicons (two). According to ISSR analysis from leaf, five out of eight ISSR markers gave 23 products with an average of 4.6 per primer. Both primers ISSR HB-12 and ISSR HB-14 appeared the highest number of bands (six). Contrarily, primer HB-11 showed the lowest number of amplicons (two). For pigeon pea seed, five out of eight ISSR markers scored 20 amplified fragments, with a mean of four per primer. Primer ISSR-89B showed the maximum number of amplicons (six). In contrast, both primers ISSR-14A and ISSR-44B, revealed the minimum number of amplicons (three). Therefore, RAPD and ISSR loci are considered diagnostic markers for the pigeon pea plant. | ||
| Keywords | ||
| DNA-based markers; molecular markers; pigeon pea | ||
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