In Silico Analysis and Experimental Validation of Two New Competing Endogenous RNA Networks in Bladder Cancer | ||
| Egyptian Journal of Chemistry | ||
| Articles in Press, Accepted Manuscript, Available Online from 17 September 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2025.390474.11846 | ||
| Authors | ||
| Mohamed Nassar1; Marwa Matboli2; Ezar Hafez3; Mahmoud Zakaria1; Ahmed Masoud3; Hazem Saleh1; Manar E Elkady* 1; Mohamed Kamel Hassan4; Bedeir Ali-El-Dein1 | ||
| 1Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Egypt. | ||
| 2Biology Department, Faculty of Medicine, Ain Shams University, Cairo 11382, Egypt. | ||
| 3Zoology Dept., Faculty of Science, Tanta University, Egypt. | ||
| 4Zoology department, Faculty of Science, Port Said university, Port Said, Egypt | ||
| Abstract | ||
| Abstract—Early and non-invasive diagnosis of bladder cancer (BC) remains a clinical challenge. The objective is to try to use in silico study to find novel competing endogenous RNA network/s (ceRNA) and to validate the findings using tissue and urine samples from a pilot cohort of BC patients (n=20), along with urine samples from 20 controls (10 healthy volunteers and 10 patients with lithiasis). Our in silico results found miR-1183 and miR-3714 as BC specific miRNAs that potentially target the tumor suppressor gene MYH9, which is involved in actin regulation. Moreover, another network revealed miR-4429 and miR-4752 which may target the other tumor suppressor, WIPF3. Additionally, LINC00670 was found to be involved in both networks. qPCR analysis of BC tissues, compared to adjacent non-cancerous tissues, showed significantly higher expression of miR-1183 and miR-3714 (p < 0.05) and a corresponding decrease in MYH9 expression (p < 0.01). Comparing urine samples from BC to those from control individuals, the same pattern was seen. In contrast, BC tissues showed reduced expression of WIPF3 (p < 0.01) and no change in expression of both miR-4429 and miR-4752 compared to adjacent normal tissues. When comparing urine samples from BC to those from control individuals, the same trend was observed (p < 0.05). Interestingly, LINC00670 was significantly upregulated in both BC tissues and urine samples (p < 0.05 and p < 0.01) compared to non-cancerous counterparts. Together, these findings suggest two novel ceRNA networks that may serve as potential biomarkers for the molecular diagnosis of bladder cancer. | ||
| Keywords | ||
| Keywords: Bladder cancer (BC); Competitive endogenous RNA (CeRNA); long non-coding RNA; micro-RNA; CeRNA net work | ||
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