Isolation and identification of Fusarium ssp. in kidney failure patients and their effects on certain immunological traits | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 19 September 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.403956.3020 | ||
| Authors | ||
| Duaa Hamada Salim* 1; Milad Adnan Mezher* 2; Maan Hasan Salih* 1 | ||
| 1Department of Biology, College of Sciences, Tikrit University, Iraq | ||
| 2Department of Biology, College of Education for Pure Sciences, Tikrit University, Iraq | ||
| Abstract | ||
| Background: Chronic Kidney Disease (CKD) is a non-reversible decline in kidney function that ultimately leads to end-stage renal disease (ESRD) requiring renal replacement therapy (such as transplantation or dialysis). Fungi, along with bacterial and viral pathogens, playing an important role in rising the mortality and morbidity associated with opportunistic infections in kidney transplant recipients. This study was designed to identify Fusarium SSP. and estimation immunological effect on kidney failure patients. Methods: This study included 100 patients with kidney failure and 30 healthy individuals (control group). Isolates were identified macro-biologically by petri dishes diffusion method. For more confirmed, the isolates microbiologically tested by glass slide technique. Immune biomarkers were estimated using ready-made. Results: The results revealed three Fusarium species: F. foetens, F. robustum, and F. luffae, which were identified microscopically and cultured in the Laboratory to confirm their species. Their effects on immune variables (IL-6, IL-10, IL-1β, and Dectin-1) were also studied. The data showed that F. robustum have had the highest significant values across all variables, with means of 0.831, 0.805, 0.692, and 0.873 picograms/mL for IL-6, IL-10, IL-1β, and Dectin-1, respectively. Overall, the results revealed no significant effect of chronic diseases on the immune variables in kidney failure patients. The results indicated a significant effect of sex on IL-10 and IL-1β levels only, with females exhibiting higher means of 0.653, 0.673, and 0.586 picograms/mL for IL-6, IL-10, and IL-1β, respectively, while males had the highest mean in Dectin-1, reaching 0.752 picograms/mL. Conclusion: F. foetens, F. robustum, and F. luffae, are the most common species that involved with chronic kidney disease, which playing important role in elevating immune biomarkers among women than men. | ||
| Keywords | ||
| Keyword: Fusarium; Kidney Failure; Immunological | ||
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