A Comparative Study on the Effects of Lactobacillus Acidophilus, Chlorella and Esomeprazole on Indomethacin-Induced Gastric Ulcer in Adult Albino Rats | ||
| Fayoum University Medical Journal | ||
| Volume 15, Issue 3, September 2025, Pages 128-155 PDF (2.12 M) | ||
| Document Type: Review Articles | ||
| DOI: 10.21608/fumj.2025.355539.1454 | ||
| Authors | ||
| Nehad Ahmed Sadek1; Dalia Amir Salah Sayed* 1; Noha Abdellatif Ibrahim1; Mohamed Mahmoud Abdellah2; Waleed Mahmoud Ragab3; Maryham George Loka1 | ||
| 1Histology and cell Biology, faculty of medicine Fayoum university, Fayoum, Egypt | ||
| 2Pathology Department, Faculty of Medicine, Fayoum University, Egypt Pathology Department, Faculty of Medicine, Galala University, Attaka, Suez, Egypt | ||
| 3Anatomy and Embryology Department, Faculty of Medicine, Fayoum University, Egypt. Anatomy and Embryology Department, Faculty of Medicine, Galala University, Attaka, Suez, Egypt | ||
| Abstract | ||
| Introduction:Gastric ulcers are among the most prevalent gastrointestinal disorders. Both Lactobacillus acidophilus and Chlorella contain natural antioxidants and anti-inflammatory agents. Aim of the study: To investigate the possibility of protective properties of Chlorella and Lactobacillus acidophilus against indomethacin-induced stomach ulcers in adult albino rats compared to usual treatment (Esomeprazole). Methods:Five groups were created from 25 mature male albino rats: Groups I (control) and II (only indomethacin): Oral 100 mg/kg, Group III (Esomeprazole + Indomethacin): 5 mg/kg Esomeprazole orally,Group IV (Chlorella + Indomethacin): 2000 mg/kg Chlorella orally, Group V (Lactobacillus acidophilus + Indomethacin): 10 billion CFU/rat orally. For ten days in a row, all rats in the experimental groups got their preventative medications, then on day 11, they were all given a single oral dosage of indomethacin. Six hours after indomethacin administration, animals were euthanized, and their stomachs were harvested for ulcer index calculations, immunohistochemical labeling (NF-κB) and histological analysis (PAS reaction, H&E staining). Morphometric analysis (area percentage of PAS and NF-κB was performed, followed by statistical evaluation. Results: When compared to control, the intake of indomethacin led to a significant increase in NF-κB expression, a significant decrease in PAS reactivity, and greater ulcer indicators. These disturbances were considerably lessened by pre-treatment with Esomeprazole, Chlorella, or Lactobacillus acidophilus. Interestingly, the most noticeable morphometric and histological improvements were seen by the Lactobacillus acidophilus group. Conclusions:Chlorella and Lactobacillus acidophilus offered substantial protective effects against indomethacin-induced gastric mucosal damage, exceeding those seen with Esomeprazole alone. Lactobacillus acidophilus exhibited a stronger prophylactic impact than Chlorella. | ||
| Keywords | ||
| Gastric ulcer; Chlorella; Lactobacillus acidophilus; Esomeprazole; NF-κB | ||
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