Positive Impact of Synbiotic Microcapsules on Immune Response and Microbial Community Modulation in Litopenaeus vannamei Challenged with Vibrio parahaemolyticus | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Volume 29, Issue 5, September and October 2025, Pages 889-907 PDF (710.5 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2025.379981.5768 | ||
| Authors | ||
| MUNTI YUHANA* 1; Abdullah Al Furqon2; Widanarni Widanarni1; Usamah Afiff3 | ||
| 1Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java, Indonesia 16680 | ||
| 2Aquaculture Science, Post Graduate Study Program, Faculty of Fishery and Marine Sciences, IPB University, Darmaga Campus, Bogor, West Java, Indonesia | ||
| 3Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, West Java, Indonesia | ||
| Abstract | ||
| This study assessed the protective efficacy of dietary multispecies synbiotic microcapsules, administered at different concentrations, in mitigating Vibrio parahaemolyticus infection in white shrimp (Litopenaeus vannamei). Juvenile shrimp, averaging 1.54 ± 0.03g in body weight, were randomly distributed into five treatment groups, each with three replicates, including a negative control (K−) and a positive control (K+), and three groups receiving synbiotic supplementation at varying levels (SIN 0.5%, SIN 1%, and SIN 1.5%). Over a 30-day period, the shrimp were given their respective synbiotic-enriched diets five times daily. On the 31st day, all groups, except the negative control, were exposed to V. parahaemolyticus at a concentration of 10⁶ CFU mL⁻¹. The synbiotic supplement has consisted of mannan oligosaccharides (MOS) as a prebiotic, along with two probiotic strains: Bacillus cereus (BR2) and Pseudoalteromonas piscicida (1UB). The findings showed that synbiotic treatment significantly (P<0.05) enhanced immune responses, promoted beneficial intestinal colonization by probiotics, reduced Vibrio parahaemolyticus levels, and decreased intestinal tissue damage as indicated by histopathological scoring. Overall, the data suggested that dietary supplementation with synbiotic microcapsules improved immune competence and reduced pathogen impact in Litopenaeus vannamei, with the 1.5% dosage identified as the most effective. | ||
| Keywords | ||
| Aquaculture; Microcapsules; Synbiotic; Vibrio parahaemolyticus; White shrimp | ||
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