Molecular Evidence and Phylogenetic Analysis of the Mitochondrial COI Gene for Species Confirmation of Mitre Squid Uroteuthis chinensis (Gray, 1849) from Bangka Island | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Volume 29, Issue 5, September and October 2025, Pages 963-977 PDF (389.67 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2025.421154.6527 | ||
| Authors | ||
| Robin Robin* 1; Tiara Puspa Anjani2; Susi Watina Simanjuntak3 | ||
| 11 Department of Aquaculture, Agriculture, Fisheries and Marine Faculty, Universitas Bangka Belitung, Jl Kampus Terpadu UBB, Balunijuk 33127, Bangka Belitung, Indonesia. | ||
| 2Department of Aquaculture, Faculty of Agriculture, Fisheries and Marine, Universitas Bangka Belitung, Jl Kampus Terpadu UBB, Balunijuk 33127, Bangka Belitung, Indonesia. | ||
| 3Department of Capture Fisheries, Faculty of Agriculture, Fisheries and Marine, Universitas Bangka Belitung, Jl Kampus Terpadu UBB, Balunijuk 33127, Bangka Belitung, Indonesia. | ||
| Abstract | ||
| Bangka Island is recognized as one of the key production areas for premium-quality squid, renowned for its superior flavor and higher market value in both domestic and international markets. Accurate species identification is essential to ensure the commercial value of this resource while supporting sustainable fisheries management. This study aimed to analyze the mitochondrial cytochrome c oxidase subunit I (COI) gene sequences of U. chinensis collected from Bangka Island, Indonesia, using DNA barcoding for species confirmation. Fresh mantle tissue samples were extracted using the Geneaid Genomic DNA Extraction Kit, followed by PCR amplification with universal primers LCO1490 and HCO2198, and bidirectional sequencing. The resulting 658 bp COI fragment exhibited 99–100% sequence similarity with reference U. chinensis sequences available in GenBank. Phylogenetic analysis using the neighbor-joining method (Kimura 2-parameter model) clustered the Bangka specimens within the U. chinensis clade with high bootstrap support (99%). These findings confirm the species identity of U. chinensis from Bangka Island and provide essential molecular data for broodstock assessment, particularly to support breeding programs and the sustainable management of squid resources. | ||
| Keywords | ||
| DNA barcoding; COI gene; U. chinensis; Species identification; Bangka Island; Phylogenetic analysis | ||
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