Phylogeographic Connectivity of Osteochilus spilurus (Cyprinidae) from Bangka, Belitung, and Kalimantan Islands Using DNA Barcoding
kurniawan, A., Hariati, A., Wiadnya, D., Kurniawan, A., Kurniawan, N., Kusuma, W. (2025). Phylogeographic Connectivity of Osteochilus spilurus (Cyprinidae) from Bangka, Belitung, and Kalimantan Islands Using DNA Barcoding. EKB Journal Management System, 29(5), 1145-1159. doi: 10.21608/ejabf.2025.421309.6529
ardiansyah kurniawan; Anik Martinah Hariati; Dewa Gede Raka Wiadnya; Andi Kurniawan; Nia Kurniawan; Wahyu Endra Kusuma. "Phylogeographic Connectivity of Osteochilus spilurus (Cyprinidae) from Bangka, Belitung, and Kalimantan Islands Using DNA Barcoding". EKB Journal Management System, 29, 5, 2025, 1145-1159. doi: 10.21608/ejabf.2025.421309.6529
kurniawan, A., Hariati, A., Wiadnya, D., Kurniawan, A., Kurniawan, N., Kusuma, W. (2025). 'Phylogeographic Connectivity of Osteochilus spilurus (Cyprinidae) from Bangka, Belitung, and Kalimantan Islands Using DNA Barcoding', EKB Journal Management System, 29(5), pp. 1145-1159. doi: 10.21608/ejabf.2025.421309.6529
kurniawan, A., Hariati, A., Wiadnya, D., Kurniawan, A., Kurniawan, N., Kusuma, W. Phylogeographic Connectivity of Osteochilus spilurus (Cyprinidae) from Bangka, Belitung, and Kalimantan Islands Using DNA Barcoding. EKB Journal Management System, 2025; 29(5): 1145-1159. doi: 10.21608/ejabf.2025.421309.6529

Phylogeographic Connectivity of Osteochilus spilurus (Cyprinidae) from Bangka, Belitung, and Kalimantan Islands Using DNA Barcoding

Egyptian Journal of Aquatic Biology and Fisheries
Volume 29, Issue 5, September and October 2025, Pages 1145-1159    PDF (430.65 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2025.421309.6529
Authors
ardiansyah kurniawan* 1; Anik Martinah Hariati2; Dewa Gede Raka Wiadnya3; Andi Kurniawan4; Nia Kurniawan5; Wahyu Endra Kusuma2
1Universitas Bangka Belitung
2Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Brawijaya
3Universitas Brawiajaya
4Department of Aquatic Resources Management, University of Brawijaya
5Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University
Abstract
Osteochilus spilurus (Bleeker, 1851), with a vast distribution in Sundaland, is one of the least utilized freshwater species. The lack of interest in this species has resulted in restricted human-caused dispersal and has offered the opportunity for a precise phylogeographic study. Fish samples were caught from six rivers on the islands of Bangka, Belitung, and Kalimantan. Fish identification was conducted using DNA barcoding of cytochrome oxidase 1 with Fish-F1 and Fish-R1 primers. Twenty-five 655 bp nucleotide sequences from this research are recorded in NCBI and represent the first such records. Bangka-Belitung and Kalimantan samples form intraspecific relationships within each region but are separated into different clades between the two regions. This separation suggests that O. spilurus may represent a cryptic species due to historical changes in the formation of the Karimata Strait between the two regions. O. spilurus has the potential to be used as a distribution and phylogeographic model for freshwater fish in Sundaland.
Keywords
Osteochilus spilurus; Sundaland; Phylogeographic; co1
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