Enhanced Cytotoxicity of Cisplatin-Loaded Mesoporous Nanoparticles Against Tongue Squamous Cell Carcinoma (HNO-97) | ||
| Egyptian Dental Journal | ||
| Volume 71, Issue 4 - Serial Number 3, October 2025, Pages 3039-3049 PDF (1.46 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2025.397941.3530 | ||
| Authors | ||
| nihal Darwish1; Doha Mohammed Afifi1; heba khaled1; Shaimaa Ali hamouda* 2 | ||
| 1Lecturer in Oral and Maxillofacial Pathology, Faculty of Dentistry, Cairo University, Egypt | ||
| 2Assistant professor in Oral and Maxillofacial Pathology, Faculty of Dentistry, Cairo University | ||
| Abstract | ||
| Cisplatin (CP) is a highly effective chemotherapeutic for tongue squamous cell carcinoma. Nanocarriers, a type of nanotechnology-based drug delivery system, have been widely employed to enhance therapeutic outcomes and limiting side effects in cancer treatment like Mesoporous silica nanoparticles (MSNs). The objective of this study is to assess CP-loaded MSNs regarding their physicochemical properties as well as their cytotoxicity in HNO-97 tongue cancer cells to evaluate their potential as a drug delivery system. Methods: A novel preparation of CP-loaded MSNs were assessed for zeta potential,particle size and polydispersity index (PDI) using dynamic light scattering (DLS) andtransmission electron microscopy (TEM). Cytotoxicity was measured using the sulforhodamine B test. The efficacy of free and CP-loaded MSNs was assessed by calculating IC 5 0 values. The analysis was performed by means of ANOVA. Results: Dynamic light scattering (DLS) analysis indicated the successful production of nanoparticles with an average size of 404.3 ± 23.1 nm, a zetapotential of -34.6 ± 0.551 mV and a polydispersity index (PDI) of 0.445 ± 0.012. In cytotoxicity experiments, the effect of CP was evident with a dosedependent decline in cell viability with the highest concentration of 100ug/ml inwhich CP-loaded MSNs were more cytotoxic (0.185±0.23) than free CP(4.50±1.26). The IC₅₀ of MSNs loaded with CP was 1.41 µg/mL versus 2.18 µg/mL obtained with free CP, suggesting higher pharmacological efficacy. Conclusions: These findings suggest that MSNs can improve the therapeutic efficacy of cisplatin through enhanced cytotoxicity, making them a potential option for CP administration, pending further in vivo validation. | ||
| Keywords | ||
| Mesoporous silica nanoparticles; cisplatin; tongue squamous cell carcinoma; drug delivery | ||
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