Upregulation of Long Non-coding RNA HOTAIR in Oral Squamous Cell Carcinoma, Oral Potentially Malignant Lesions Versus Control Patients: A Case-Control Study | ||
| Egyptian Dental Journal | ||
| Volume 71, Issue 4 - Serial Number 3, October 2025, Pages 3127-3136 PDF (285.89 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2025.411991.3590 | ||
| Authors | ||
| Ahmed Adel Abdel-Azim1; Olfat Gamil shaker2; Noha Ahmed El-Sherif3; Alaa El-Deen Ragab Mahmoud* 4; Noha Nasr Mohamed5 | ||
| 1Lecturer of Oral Medicine and Oral Diagnosis- Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dentistry, Menoufia University, Egypt. | ||
| 2Professor of Medical Biochemistry, Faculty of Medicine, Cairo University, Egypt. | ||
| 3Lecturer of Oral biology, Faculty of Oral and Dental Medicine, Egyptian Russian University | ||
| 4Lecturer of Oral and Maxillofacial Surgery, Faculty of Dentistry, Menoufia University, Egypt. | ||
| 5Lecturer of Oral Medicine and Oral Diagnosis- Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dentistry, Modern University For Technology and Information, Egypt. | ||
| Abstract | ||
| Oral squamous cell carcinoma (OSCC) is one of the most common tumors in the head and neck area. Recent research suggests that the increased expression of the long non-coding RNA (lncRNA) HOTAIR correlates with the progression of many cancer types. The aim of this study: The objective of this research was to ascertain if levels of long non-coding RNA HOTAIR in saliva are advantageous and correspond among the oral squamous cell cancer (OSCC) group, the oral potentially malignant lesions (OPMLs) group, and the control group. Materials & Methods: A total of 45 participants (n=15) were evenly allocated into three distinct groups. Group A, diagnosed with OSCC; Group B, diagnosed with OPMLs; and Group C, the control group. Saliva samples were collected from participants through unstimulated spitting and subsequently preserved in sterile vials. The extraction of LncRNA HOTAIR from saliva was conducted, followed by preparation for genetic analysis utilizing Reverse Transcription (RT) and real-time Quantitative PCR (qPCR). Results: No statistically significant variations were found between the groups concerning gender; nevertheless, highly statistically significant disparities were noted between the median ages of the control group and those of the premalignant and OSCC groups. Concerning HOTAIR expression, a significantly significant disparity was seen between the OSCC and control groups, however no significant difference was noted between the OPMLs and control groups. Conclusions: The expression of HOTAIR is linked to OSCC and may serve as a crucial target in its evolution. Nonetheless, HOTAIR is not implicated in the tumor progression of OPMLs. | ||
| Keywords | ||
| HOTAIR; oral squamous cell carcinoma; oral potentially malignant lesions; long noncoding RNAs | ||
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