Modulation of Cellular Stress Response by Matcha Tea Extract in E-Cigarette-Exposed Cells: In Vitro Investigations | ||
| Ain Shams Dental Journal | ||
| Volume 38, Issue 2, June 2025, Pages 229-239 PDF (1.26 M) | ||
| Document Type: Original articles | ||
| DOI: 10.21608/asdj.2024.320730.1516 | ||
| Authors | ||
| Hala El-kammar* 1; hend El-messiry2; Aya Magdy3; Iman Mostafa Abelmeguid4 | ||
| 1Villa 103, Street 17, 5th Settlement, New Cairo, Cairo, Egypt | ||
| 2Associate Professor, Department of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt. | ||
| 3Assistant lecturer, Oral and maxillofacial surgery, Faculty of Oral and Dental Medicine, Future University in Egypt, Cairo, 11835, Egypt; | ||
| 4oral pathology department, future university in Egypt, Cairo, Egypt | ||
| Abstract | ||
| Aim: Matcha, a vibrant green tea powder has gained popularity as a trendy beverage due to its potent antioxidant properties. E-cigarettes, despite increasing consumption, especially among young adults, have been linked to health deterioration. This study investigated the potential of matcha tea extract to mitigate the harmful effects of e-cigarette liquid on human fibroblast cells. Materials and methods: Human fibroblast cells were divided into four groups: control, matcha tea extract only, e-cigarette liquid only, and a combination of both. Cytotoxic concentrations were determined by MTT assay, and cell morphology, death, cycle, protein expression (Bax/Bcl2), and ultrastructure were analyzed. Results: Flowcytometry analysis revealed a decrease in necrotic and apoptotic cell populations in cells treated with both matcha tea extract and e-cigarette liquid compared to those exposed to e-cigarette liquid only. Ultrastructural examination revealed that necrotic morphology was most prominent in the e-cigarette liquid only group. Cell cycle analysis indicated a slightly higher accumulation of cells in the G2/M phase in the e-cigarette liquid only group compared to the combination group, while the combination group had a higher percentage of cells in the S phase. Conclusion: While matcha tea extract may not be a complete solution, it offers promising insights into the potential of natural compounds to mitigate the harmful effects of e-cigarettes. Further research is essential to fully understand its mechanisms of action and explore its clinical relevance. | ||
| Keywords | ||
| Electronic Cigarettes e-Liquid; Matcha; reactive oxygen species; cell death; cell cycle | ||
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