Evaluation of cytotoxicity and the antibacterial effect of Salvia officinalis as an intra-canal medicament using Confocal microscope | ||
| Ain Shams Dental Journal | ||
| Volume 38, Issue 2, June 2025, Pages 283-294 PDF (1.24 M) | ||
| Document Type: Original articles | ||
| DOI: 10.21608/asdj.2025.356725.1829 | ||
| Authors | ||
| Abdallah A. Atris* 1; Yousra Mohamed Nashaat2; Maram Farouk Obeid3; Rasha Sameh Mahfouz4 | ||
| 1Endodontics Department, Faculty of Dentistry, October 6 University, 6th of October, Egypt | ||
| 2Professor of Endodontics, Endodontics Department, Faculty of Dentistry, October 6 University, 6th of October, Egypt | ||
| 3Professor of Endodontics, Endodontics Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt | ||
| 4Associate Professor of Endodontics, Endodontics Department, Faculty of Dentistry, October 6 University, 6th of October, Egypt | ||
| Abstract | ||
| Aim: The principal objective of root canal therapy is to control the infection in the root canal system after mechanical preparation and irrigation by eliminating the current infection and preventing any future recurrence. The present study investigated the antibacterial efficacy and cytotoxicity of Salvia officinalis (SOL) extract and its nanoform (SOL-NPs) as intracanal medicaments against Enterococcus faecalis, compared to calcium hydroxide. Materials and methods: Cytotoxicity of SOL extract and SOL-NPs against the normal human epithelial cell line 1-BJ1 (Normal skin fibroblast) using MTT assay. 70 teeth were collected and evenly split into three experimental groups (n=20): A, B, and C, and one negative control group (n=10). Group A teeth were injected with ready-to-use Metapaste calcium hydroxide. Group B received S. officinalis extract paste injections. roup C teeth received nanoform S. officinalis paste injections. The negative control samples were infected with root canal-isolated Enterococcus faecalis. Results: The IC50 values were 594 µg/ml for SOL extract, 1468.8 µg/ml for SOL-NPs, and 1219 µg/ml for Metapaste, highlighting the SOL extract as the most potent. Both the SOL extract and SOL-NPs showed significant antibacterial activity against E. faecalis, with SOL-NPs proving more effective than CH in reducing bacterial counts. Conclusion: SOL extract and SOL-NPs effectively reduced bacterial cell counts and dead cells, with SOL-NPs showing superior antibacterial properties, potentially treating Enterococcus faecalis in intracanal settings. The findings underscore the potential of plant-based solutions in managing dental infections while minimizing cytotoxic risks. | ||
| Keywords | ||
| Intracanal medicaments; Salvia officinalis; Nanoparticles; Cytotoxicity; Antibacterial inhibition | ||
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