Employment of Tissue Culture Techniques in Improvement Propagation of Paulownia Tomentosa Plant | ||
| Journal of Plant Production | ||
| Article 12, Volume 7, Issue 6, June 2016, Pages 619-625 PDF (547.59 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2016.45543 | ||
| Author | ||
| Y. Ghatas* | ||
| Horticulture, Dept. Fac. of Agric., Benha University, Egypt. | ||
| Abstract | ||
| Different media and explant types, anti-oxidant pre-treatments, additives, cytokinin types &concentrations as well as medium strength, GA3 concentrations, auxin types &concentrations were studied to find out the best protocol of in vitro propagation of Paulownia tomentosa. The results indicated that culturing of pre-treated shoot tips with anti-oxidant solution on modified Murashige&Skoog medium supplemented with PVP and activated charcoal as anti-oxidants, as well as adenine sulphate and coconut milk as additives maximized survival percentage and improved explant development. Also, using of 2mg/ L BAP increased proliferation. Addition of 1.0 mg/ L GA3 to half strength medium maximized shoot length. Moreover, addition of 1.0 IBA to the culture medium encouraged the highest shoot length and number of roots. | ||
| Keywords | ||
| In vitro culture; Paulownia tomentosa; Woody plants | ||
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