Histochemical studies of Mesenchymal Stem Cells Effect on Possible Adverse Effects of Rapamycin Drug; on Lymph Node and Peyer's Patches in Adult Male Albino Rats | ||
| Al-Azhar International Medical Journal | ||
| Volume 2025, Issue 5, May 2025, Pages 257-263 PDF (1.06 M) | ||
| Document Type: Original Article | ||
| DOI: https://doi.org/10.21608/aimj.2025.446588 | ||
| Authors | ||
| Elsayed Saad Hamed* 1; Ashraf Mahmud Mustafa2; Rania Abd Elazim Galhom3; Mohamed Sobhy Nasr2 | ||
| 1Histology Department, Faculty of Medicine, Arish University, EgyptHistology Department, Faculty of Medicine, Arish University, Egypt | ||
| 2Histology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt | ||
| 3Anatomy Department, Faculty of Medicine, Suez Canal University, Egypt | ||
| Abstract | ||
| Background: The possible effects of Bone marrow-derived mesenchymal stem cells (BM-MSCs) on the possible adverse effects of immunosuppressive drugs, which are commonly used nowadays. In this study, we assess the therapeutic potential effect of BM-MSCs on adverse effects induced by rapamycin overdose treatment by evaluating the histology and functional integrity of lymph nodes and Peyer's patches under amplified BM-MSCs /or rapamycin treatments compared with the control group. The present study was carried out on 40 healthy albino rats of different age groups. The rats were divided into four groups. Analytical methods include flow cytometry, ELISA for cytokine detection, and histological staining protocol assessments. Results: Findings indicated that as rapamycin concentrations increased, BM-MSC viability decreased. Histological evaluations revealed improved structures in lymph nodes and Peyer's patches when BM-MSCs were co-administered with rapamycin, suggesting that BM-MSCs can counteract rapamycin's immunosuppressive effects. Conclusion: Integrating BM-MSC therapy could enhance its therapeutic safety profile, making it a viable approach for reducing the adverse effects of immunosuppressive regimens in clinical practice. | ||
| Keywords | ||
| Rapamycin; BM-MSCs; lymph node; Adverse effects; Peyer's Patches | ||
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