The Anticancer Effect of Piperine on Tongue Squamous Cell Carcinoma Cell Line | ||
| Ain Shams Dental Journal | ||
| Volume 38, Issue 2, June 2025, Pages 394-401 PDF (1.13 M) | ||
| Document Type: Original articles | ||
| DOI: 10.21608/asdj.2024.304680.1377 | ||
| Authors | ||
| Manar Ali Mohamed* 1; Sherif Farouk El-Gayar2; Enas Alaa Eldin Abd Elaziz2 | ||
| 1Assistant lecturer of Oral and Maxillofacial pathology, Sphinx University | ||
| 2Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University | ||
| Abstract | ||
| Aim: The purpose of this study was to investigate the impact of piperine (PP) on tongue squamous cell carcinoma cell line. Materials and Methods: In this research, cultured tongue carcinoma cells were separated into two groups: a control group, and PP treated group. The cytotoxicity assay was utilized to measure cell viability. Flow cytometry was employed to examine and assess the cell cycle and apoptosis phases. Results: Piperine induced cytotoxicity on tongue carcinoma cells, reducing the mean percentage of cell viability in a concentration-dependent manner, with an IC50 of 21.2 μM. Moreover, PP treatment induced a rise in the percentage of cells in the G1 to S phases of the cell cycle and a reduction in the percentage of cancer cells in the G2/M phase. Exposure of tongue carcinoma cells to PP led to a decrease in the number of viable cells, along with a rise in the combined proportion of apoptotic and necrotic cells to 31.14%. Conclusion: Piperine reduced the viability, caused cell cycle changes, and induced cell death of tongue squamous cell carcinoma cell line suggesting that PP has a potential as a therapeutic agent. | ||
| Keywords | ||
| Piperine; Carcinoma; Cell cycle analysis; Tongue | ||
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