Azithromycin-Loaded Solid Lipid Nanoparticles as a nanodrug delivery Strategy against Multidrug-Resistant Escherichia coli: Preparation, Characterization, and In-vitro Evaluation
El-Mogy, A., Omer, A., Ateya, A., A. Hasaneen, M. (2025). Azithromycin-Loaded Solid Lipid Nanoparticles as a nanodrug delivery Strategy against Multidrug-Resistant Escherichia coli: Preparation, Characterization, and In-vitro Evaluation. EKB Journal Management System, 72(3), 16-26. doi: 10.21608/mjb.2025.455958
Amany I. El-Mogy; Aya M. Omer; Ahmed I. Ateya; MohammedNagib A. Hasaneen. "Azithromycin-Loaded Solid Lipid Nanoparticles as a nanodrug delivery Strategy against Multidrug-Resistant Escherichia coli: Preparation, Characterization, and In-vitro Evaluation". EKB Journal Management System, 72, 3, 2025, 16-26. doi: 10.21608/mjb.2025.455958
El-Mogy, A., Omer, A., Ateya, A., A. Hasaneen, M. (2025). 'Azithromycin-Loaded Solid Lipid Nanoparticles as a nanodrug delivery Strategy against Multidrug-Resistant Escherichia coli: Preparation, Characterization, and In-vitro Evaluation', EKB Journal Management System, 72(3), pp. 16-26. doi: 10.21608/mjb.2025.455958
El-Mogy, A., Omer, A., Ateya, A., A. Hasaneen, M. Azithromycin-Loaded Solid Lipid Nanoparticles as a nanodrug delivery Strategy against Multidrug-Resistant Escherichia coli: Preparation, Characterization, and In-vitro Evaluation. EKB Journal Management System, 2025; 72(3): 16-26. doi: 10.21608/mjb.2025.455958

Azithromycin-Loaded Solid Lipid Nanoparticles as a nanodrug delivery Strategy against Multidrug-Resistant Escherichia coli: Preparation, Characterization, and In-vitro Evaluation

Mansoura Journal of Biology
Volume 72, Issue 3, September 2025, Pages 16-26    PDF (1.16 M)
Document Type: Original Article
DOI: 10.21608/mjb.2025.455958
Authors
Amany I. El-Mogy1; Aya M. Omer1; Ahmed I. Ateya2; MohammedNagib A. Hasaneen* 1
1Botany Department, Faculty of Science, Mansoura University.
2Department of Development of Animal Wealth, Faculty of Veterinary, Mansoura University
Abstract
The global rise of multidrug-resistant bacteria has intensified the demand for new antimicrobial agents and advanced delivery systems. This study aimed to evaluate the synergistic antibacterial activity and underlying mechanisms of azithromycin (AZM)-loaded solid lipid nanoparticles (SLNPs) against Escherichia coli. SLNPs were synthesized via the hot homogenization technique and subsequently encapsulated and loaded with AZM to assess release kinetics and antibacterial efficacy. Physicochemical characterization of both blank and AZM-loaded SLNPs was conducted using transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), zeta potential analysis, and in-vitro antibacterial assays. The results revealed that the SLNPs exhibited a spherical morphology, and their particle size increased by approximately 61.26% upon AZM loading. In-vitro release studies demonstrated a sustained and controlled drug release profile, reaching 37.2% over 12 hours. Importantly, AZM-loaded SLNPs at 50 mg ml-1 exhibited a 9.01% higher antibacterial activity against E. coli compared to conventional AZM formulations, indicating improved efficacy and potential for dose reduction. These findings highlight the promising role of SLNPs as a nanocarrier platform to enhance the therapeutic performance of azithromycin in combating E. coli infections. In conclusion, solid lipid nanoparticles serve as an effective nanodrug delivery system to improve the antibacterial action of azithromycin in a dose-dependent manner, offering a potential strategy to combat antibiotic resistance in Gram-negative bacteria such as E. coli.
Keywords
Azithromycin; drug delivery; E. coli; solid lipid nanoparticles
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