Evaluation of Nannochloropsis oculata as Potential Feedstock for Sustainable Biodiesel Production | ||
Mansoura Journal of Biology | ||
Volume 72, Issue 3, September 2025, Pages 27-37 PDF (1.32 M) | ||
Document Type: Original Article | ||
DOI: 10.21608/mjb.2025.455960 | ||
Authors | ||
Islam Mamdouh1; Emad H. El-Bilawy2; Eladl Eltanahy1; Jelan Mofeed3 | ||
1Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt | ||
2Faculty of Basic Sciences, King Salman International University, South Sinai, Egypt | ||
3Aquatic Environment Department, Faculty of Fish Resources, Suez University, Suez, Egypt | ||
Abstract | ||
The increasing global focus on renewable and sustainable energy sources has highlighted microalgae as a promising feedstock for biodiesel production. This study evaluates the potential of Nannochloropsis oculata, a green alga known for its high lipid content, in producing environmentally friendly biodiesel. The microalga was cultured in 2 medium under controlled environmental conditions to analyze its growth behavior, biomass yield, and lipid accumulation. A distinct sigmoidal growth curve was observed, with the stationary phase reached at day 25 and a baseline lipid content was recorded 19.8%. Further experimental modifications, particularly adjusting the nitrogen-to-phosphorus (N:P) ratio and light exposure, led to notable improvements in both biomass and lipid production, achieving a maximum lipid content of 30.91% under a 1:1 N:P ratio. GC-MS profiling identified major fatty acids such as palmitic acid (C16:0), oleic acid (C18:1), and stearic acid (C18:0), which are crucial for optimal biodiesel characteristics. Using Biodiesel Analyzer software, key fuel properties were predicted, including a high cetane number (97.65) and a low cold filter plugging point (-2.72°C), meeting international biodiesel standards. These findings d that Nannochloropsis oculata holds strong potential as a sustainable and efficient source for biodiesel, offering a carbon-neutral alternative that addresses both ecological and economic challenges. | ||
Keywords | ||
Nannochloropsis oculata; Biodiesel; Microalgae; Lipid content; Renewable energy | ||
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