Advantage of free beta carotene over its liposomal form in restraining the growth of radiation-exposed breast carcinoma cell line (MCF-7) | ||
| Arab Journal of Nuclear Sciences and Applications | ||
| Volume 58, Issue 4, October 2025, Pages 1-16 PDF (1.36 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajnsa.2025.396413.1890 | ||
| Authors | ||
| Wael M Elshemey* 1; Doha Ahmed2; Yasser Y Eaid2; Moustafa Shaban3; Medhat Shafaa4 | ||
| 1Physics Department, Faculty of Science Islamic University of Madinah Abo Bakr Al Siddiq road, Al Jamiah, Madinah | ||
| 2Physics Department, Faculty of Science, Fayoum University, Fayoum, EGYPT | ||
| 3Department o Physics and Engineering Mathematics, Higher Institute of Engineering, El-Shorouk Academy, El-Shorouk City, Cairo, EGYPT | ||
| 4Physics Department, Faculty of Science, Helwan University, Cairo, EGYPT | ||
| Abstract | ||
| This work aimed to provide an in-depth study of the interaction between β-carotene and lecithin liposomes. The study additionally investigated the toxicity of beta-carotene, in both its unencapsulated and liposome-bound forms, on MCF-7 breast cancer cells that had been subjected to radiation and those that had not. The interactions between β-carotene and lecithin liposomes, serving as model membranes, were analyzed. Liposomes exhibited a predominantly well dispersed, spherical morphology with minimal aggregation. Reduced zeta potential values were observed after β-carotene was incorporated into the liposomal membranes, indicating a change in surface charge. Furthermore, incorporating β-carotene caused a decrease in the temperatures observed, in contrast to empty liposomes, suggesting a conformational change within the phospholipids. This was confirmed by FTIR studies, which demonstrated interactions between β-carotene and the liposomal components. In cytotoxic assays with MCF-7 cells, if external gamma irradiation was withheld, the IC50 value for free β-carotene was 3.45 μg/ml. In contrast, liposomal β-carotene exhibited a higher IC50 of 52.30 μg/ml. When combined with gamma irradiation at 5 Gy followed by 10 Gy, the IC50 values for free β-carotene and liposomal β-carotene increased slightly, from 3.14 to 4.05 μg/ml and from 50.21 to 53.92 μg/ml, respectively. The data suggests free β-carotene's cytotoxic effect is more powerful than that of liposomal β-carotene. The study emphasizes the potential of free β-carotene as an effective anticancer agent against the MCF-7 cell line. These results advocate for considering the natural product, free β-carotene, as a promising alternative to pharmacological interventions in breast carcinoma treatment. | ||
| Keywords | ||
| β-carotene; Liposomes; FTIR; MCF-7; Cytotoxicity; Gamma Irradiation | ||
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