Effect of n-acetyl cysteine and moringa oleifera on acute kidney injury induced by cisplatin in rats. | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Volume 45, Issue 4, October 2025, Pages 532-549 PDF (908.53 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2025.383383.1214 | ||
| Authors | ||
| Eman mahmoud Abd elhameed* 1; Zeinab halim1; Mohamed Adel1; Aya E. Abd El-Hamed2; Refka Khalil messiha1 | ||
| 1Medical Physiology Department, Faculty of Medicine, Mansoura University, Egypt | ||
| 2Medical Experimental Research Center (MERC), Mansoura University,Mansoura, Egypt | ||
| Abstract | ||
| This study aimed to assess the effects of N-acetylcysteine and Moringa oleifera on cisplatin-induced acute kidney injury (AKI) in rats. Forty adult male rats were divided into five groups: Group 1 (control), Group 2 (cisplatin, 6 mg/kg IP on the 8th day), Group 3 (N-acetylcysteine, 200 mg/kg/day orally for 12 days + cisplatin), Group 4 (Moringa oleifera, 300 mg/kg/day orally for 12 days + cisplatin), and Group 5 (combined N-acetylcysteine and Moringa oleifera + cisplatin). Blood samples were collected for biochemical analysis, and renal tissue was examined for oxidative stress markers, caspase 3, and aquaporin 2 expression. Results revealed marked histopathological damage and increased caspase 3 expression in Group 2. N-acetylcysteine and Moringa oleifera treatments reduced this damage, with the best outcome in Group 5. Aquaporin 2 expression decreased significantly in Group 2 but improved in the treated groups, particularly Group 5. Serum creatinine, urea, and potassium levels increased significantly in Group 2, whereas treatment with N-acetylcysteine and Moringa oleifera significantly reduced these levels, with the best results in Group 5. Creatinine clearance decreased in Group 2 but improved with treatment, especially in Group 5. Serum KIM-1, serum NGAL, and urine NGAL were elevated in Group 2 and reduced in the treated groups, with the best outcome in Group 5. Oxidative stress markers improved in Groups 3, 4, and 5, with the best results in Group 5. A negative correlation was observed between serum KIM-1 and aquaporin 2. In conclusion, N-acetylcysteine and Moringa oleifera may protect against cisplatin-induced AKI. | ||
| Keywords | ||
| Keywords: oxidative stress; apoptosis; cisplatin; n-acetylcysteine; moringa olifera | ||
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